开发未可培养微生物技术的研究进展  

Advance in Technique on the Development of Uncultured Microorganism

作　　者：孙晓媛[1] 李影[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科技学院,长春130118

出　　处：《微生物学通报》2008年第5期798-802,共5页Microbiology China

基　　金：国家自然科学基金项目(No.30471286;No.30700594);吉林省科技发展计划项目(No.20060556)

摘　　要：细菌的"活的非可培养状态"(VBNC,viable but nonculturable)发现于20世纪80年代,处于此状态的细菌不但丧失了在培养基上生长繁殖的能力,而且具有与原菌相似的致病性,成为可以逃避检测的"隐性"传染源,对周围的环境及人类安全构成潜在威胁。作为公认的未可培养微生物,它一直是预防医学、流行病学、微生物生态学以及公共卫生检验检疫方面研究的热点问题之一。现代分子生物学技术和基因组学的深入研究,为开发环境中的未可培养微生物提供了新的研究方法和机遇。其中遗传指纹图谱技术、宏基因组技术显示出一定的优势,同时,随着各种细菌的非可培养状态的实验室模型已日臻成熟,这为开发和利用未可培养微生物资源提供了新的研究思路。Viable but non-culturable state in bacterial cells as received uncultured microorganism has become the fundamental research issues in medical microbiology, epidemiology, general microbial ecology and sanitation quarantine since it was put forward in 1982. When in this state, bacterium are no longer able to grow and form colonies on conventional culture media, but maintain their patheogenicity, may become latent infection escaping detection to threaten enviroment and human security. With the development of modern molecular biology technique and metagenomics, it provide new research method and opportunity for uncultured microorganism in the environment. Recently, with the application of the metagenome technique, genetic fingerprinting technical etc, it becomes more and more popular. Simultaneously, along with this state become maturity in the laboratory. It provides a new research route for development and exploitation the uncultured microorganism.

关 键 词：VBNC 未可培养微生物 宏基因组学 遗传指纹图谱技术 

分 类 号：Q93-3[生物学—微生物学]