气相色谱法快速测定植物油沥青中的甾醇  

Quick determination of sterols in plant oil pitch by capillary gas chromatography

作　　者：卢定强[1] 吴雅霜[1] 陈建辉[2] 段柳[1] 凌岫泉[1] 

机构地区：[1]南京工业大学制药与生命科学学院,材料化学工程国家重点实验室,江苏南京210009 [2]浙江医药股份有限公司,浙江绍兴312500

出　　处：《食品工业科技》2008年第5期268-269,共2页Science and Technology of Food Industry

基　　金：国家重大基础研究项目(2003CB716000)

摘　　要：建立了一种气相色谱法，用于快速测定植物油沥青中植物甾醇的组分和含量。方法中采用HP-5毛细管柱，FID检测器检测，内标法计算含量。甾醇在1～10mg／mL呈良好线性关系，植物油沥青中甾醇总合量为6．08％，包括菜油甾醇、豆甾醇和β-谷甾醇，回收率为96．2％-102．7％，相对标准偏差为1．54％。结果表明，该法操作简单、快速、准确。植物油沥青因富含甾醇，具有很高的应用价值。A gas chromatographic method was described for the determination of plant sterols in plant oil pitch. The sterols were analyzed by gas chromatography with HP-5 capillary column and flame ionization detection using an internal standard method. The sterols range from 1-lOmg/mL. In plant oil pitch, the total content of sterols was 6.08%, which include campesterol, stigmasterol and β-sitosterol. The recovery and RSD was 96.2%-102.7% and 1.54% respectively. The result showed that this method was simple, rapid and accurate. And the plant oil pitch is valuable for its high content of phytosterols.

关 键 词：气相色谱法 植物油沥青 甾醇 

分 类 号：TS207.3[轻工技术与工程—食品科学]