Aβ25~35合并鹅膏蕈氨酸诱导老年痴呆大鼠模型的建立及评价  

Establishment and evaluation of Aβ_(25～35) and IBO induced the aged rat model of Alzheimer′s disease

作　　者：孔明望[1] 王平[1] 田代志[1] 石和元[1] 

机构地区：[1]湖北中医学院老年医学研究所,“中药资源与中药复方”省部共建教育重点实验室,湖北武汉430065

出　　处：《中国老年学杂志》2008年第10期945-947,共3页Chinese Journal of Gerontology

基　　金：国家自然基金资助项目(No.30472249);湖北省自然科学基金计划重大项目(No.2006ABD008)

摘　　要：目的建立一种新的复合式老年痴呆（AD）大鼠模型,用于AD及其治疗药物的研究。方法选用15月龄Wistar大鼠,随机分为4组：正常老年组,Aβ25~35模型组,IBO模型组,Aβ25~35＋IBO模型组。双侧基底前脑注射制备各种老年痴呆动物模型。通过水迷宫试验,脑组织病理切片银染色、刚果红染色,比较各老年痴呆模型的差异。结果在定位航行中,Aβ25~35＋IBO组潜伏期（92.4±11.6）s比IBO组（73.6±8.4）s,Aβ25~35组（71.7±9.2）s明显增加（P〈0.01）。各模型组大鼠大脑皮层都出现类老年斑病理改变和神经元纤维缠结,但以Aβ25~35＋IBO组最明显。结论Aβ25~35合并IBO制备的老年痴呆模型在一定程度上较传统模型更好地模拟AD的发病特点,可作为AD及其治疗药物研究的一种新模型。Objective To establish a novel complex rat model of Alzheimer＇s disease （AD） for research of AD and its treatment. Methods The normal 15 months old Wistar rat were randomly divided into normal, Aβ25-35 model, IBO model [ injection of basal nucleus of Meynert （BNM） with ibotenic acid （IBO）] and Aβ25-35 ＋ IBO （accumulation Aβ25-35 and the composition of IBO） model groups. The rat water-maze test and the pathological observation with silver and congo red staining of the hippocampus and cerebral cortex were carried out to evaluate the mouse model of AD. Results The latency period in positioning navigation test in Aβ25-35 ＋ IBO group [ （ 92. 4 ±11.6 ） s was significantly higher than that in Aβ25-35 [ （73.6 ±8.4） s] and IBO [ （71.7 ± 9. 2） s] groups （ P 〈 0. 01 ）. The senile plaques-like change and neurofibrillary tangles in cerebral cortex were saw in these model groups, especially in Aβ25-35 ＋ IBO group. Conclusions The animal AD model induced by Aβ25-35 and IBO injected into basal nucleus of Meynert simulates some characteristics of human AD to some extent, which may be used as a novel model to study AD and its drug treatment.

关 键 词：阿尔茨海默病 动物模型 神经纤维缠结 老年斑 

分 类 号：R749.16[医药卫生—神经病学与精神病学]