海参营养液DNA高效快速提取及种类鉴定方法  

An Efficient and Rapid Method for DNA Extraction and Species Identification from Sea Cucumber Nutrient Solution

作　　者：梁君妮[1] 薛长湖[1] 赵玉然[1] 唐庆娟[1] 许加超[1] 杨文鸽[2] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]宁波大学生命科学与技术学院,浙江宁波315211

出　　处：《食品科学》2008年第5期269-272,共4页Food Science

基　　金：新世纪优秀人才支持计划项目(NCET-04-0642);国家自然科学基金项目(30471319;30671632)

摘　　要：本实验以海参口服液为原料,采用异丙醇和乙酸钠沉淀的改良方法提取DNA,通过A260/A280、琼脂糖凝胶电泳和PCR检测,对DNA溶液进行定性分析和综合比较。结果表明,本方法是一种新型的海参营养液DNA高效快速提取及种类鉴定的方法,可用于对海参营养液进行定性检测。Mitochondria DNA of sea cucumber were extracted from the sea cucumber nutrient solution with optimized method. The quantitative and qualitative analyses of DNA solution were performed with the calculation of A260/A280, Agarose gel electrophoresis, and PCR. The results showed that our method of DNA extraction and species identification from the sea cucumber nutrient solution was more efficient and rapid than traditional methods. This study will be helpful for quicker, more economical, and dependable species identification of the sea cucumber.

关 键 词：海参营养液 DNA提取 种类鉴定 

分 类 号：Q523[生物学—生物化学]