柿树乙醇脱氢酶活性与可溶性单宁含量的关系  

Relationship Between Activity of Alcohol Dehydrogenase and Soluble Tannin in Persimmon

作　　者：刘朝蓬[1] 郑仲明[2] 梁银娜[1] 李宝[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]北京市房山区林业局林果站,北京102400

出　　处：《园艺学报》2008年第5期741-746,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671713);中国农业大学科研启动基金项目(2005061)

摘　　要：研究了4种不同脱涩类型柿(Diospyros kaki Thunb.)果实和叶片中的可溶性单宁含量、乙醇脱氢酶(alcohol dehydrogenase,ADH)活性和ADH最适反应温度,以了解ADH在脱涩过程中的作用。结果表明:不完全甜柿和完全甜柿果实发育前期,可溶性单宁含量逐渐降低,ADH活性逐渐增高,到果实基本完成脱涩后,ADH活性又开始降低,在不完全甜柿中变化较大;涩柿和不完全涩柿在果实近熟期,可溶性单宁含量有所下降,但不能完成脱涩,且ADH活性较低。叶片发育过程中,可溶性单宁含量的变化与ADH没有相关性。ADH的最适反应温度在25℃左右。ADH与柿果实脱涩有一定关系。Content of soluble tannin and activity of alcohol dehydrogenase and the optimum temperature of alcohol dehydrogenase （ADH） in 4 different type fruits of persimmon （Diospyros kaki Thunb. ） were measured in order to assess the effects of ADH on loss of astringency of persimmon fruits. In the early stage of fruit development, the pollination-constant/nonastringent （PCNA） and the pollination-variant/nonastringent （PV- NA） soluble tannin content decreased, and the activity of ADH increased, but the activity of ADH decreased when the fruits become non-astringent, especially in PVNA-type fruits. The content of soluble tannin of pollination-constant/astringent （PCA） and pollination-variant/astringent （PVA） -type fruits decreased, and the activity of ADH was also in lower level in the mature stage. During the leaf development, there is no significant relationship between ADH and soluble tannin. The optimum temperature of ADH is 25 ℃. ADH plays a role to some extent in loss of astringency of persimmon fruits.

关 键 词：柿 可溶性单宁 乙醇脱氢酶 脱涩 

分 类 号：S665.2[农业科学—果树学]