子宫内膜样腺癌APC基因表达和甲基化的初步研究  

Expression and Methylation of Adenomatous Polyposis Coli Gene in Endometrioid Adenocarcinoma

作　　者：钱冰[1] 柯佩琪[1] 王莉[2] 刘文姬[2] 李美香[2] 

机构地区：[1]中山大学附属第一医院妇产科 [2]华南肿瘤学国家重点实验室

出　　处：《癌症》2008年第6期585-589,共5页Chinese Journal of Cancer

基　　金：子宫内膜癌CpG岛甲基化表型(CIMP)的研究(No.2005B34201022)~~

摘　　要：背景与目的:子宫内膜癌是女性生殖系统常见的恶性肿瘤之一。在我国,近年来其发病率亦显著上升。APC基因是一种抑癌基因,在很多组织中都有表达,其表达与卵巢癌有一定的关系。本实验探讨APC基因的甲基化和表达与子宫内膜样腺癌的发生发展的关系。方法:运用甲基化特异性PCR、RT-PCR、免疫组化方法检测了30例正常的增生期子宫内膜组织、30例不典型增生的子宫内膜组织、60例子宫内膜样腺癌组织中APC基因的甲基化情况以及mRNA和蛋白的表达情况。结果:子宫内膜样腺癌组织中APC基因的甲基化率(65.0%)、mRNA表达率(33.3%)及APC蛋白表达率(30.0%)与不典型增生子宫内膜组织(33.3%、63.3%、50.0%)及正常子宫内膜(23.3%、73.3%、66.7%)相比,差异均具有显著性(P<0.05);而不典型增生子宫内膜组织和正常子宫内膜组织中,APC的甲基化率、mRNA表达率及APC蛋白表达率差异无统计学意义(P>0.05)。在这3种组织中,APC基因的甲基化和mRNA的失表达呈正相关关系。结论:APC的甲基化和表达与子宫内膜样腺癌的发生发展相关。BACKGROUND ＆ OBJECTIVE： Endometrial carcinoma is a common malignant tumor of female reproductive system, with an increasing incidence in China. Adenomatous polyposis coli （APC） gene, a tumor suppressor gene, is expressed in many tissues, and has a certain relationship with ovarian cancer. This study was to observe the expression and DNA methylation of APC gene in endometrioid adenocarcinoma, and explore its correlations to the occurrence and development of this disease. METHODS： The methylation, mRNA and protein expression of APC gene were detected by methylation-specific polymerase chain reaction （PCR）, reverse transcription-polymerasechain reaction （RT-PCR） and immunohisto- chemistry in 30 specimens of normal proliferative endometrium, 30 specimens of atypical hyperplastic endometrium and 60 specimens of endometrioid adenocarcinoma. RESULTS： The methylation rate of APC gene was significantly higher, the positive rates of APC mRNA and protein were significantly lower in endometrioid adenocarcinoma than in atypical hyperplastic endometrium and normal proliferative endometrium （65.0% vs. 33.3% and 23.3%, 33.3% vs. 63.3% and 73.3%, 30.0% vs. 50.0% and 66.7% ,P〈0.05）. There was no significant difference between atypical hyperplastic endometrium and normal endometrium （P〉0.05）. APC methylation was positively correlated to APC mRNA expression. CONCLUSION： The expression and DNA methylation of APC gene are certainly related with the occurrence and development of endometrioid adenocarcinoma.

关 键 词：子宫肿瘤 内膜样腺癌 APC基因 DNA甲基化 病理学 

分 类 号：R737.33[医药卫生—肿瘤]