茶树种质资源遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Tea Cultivars Camellia sinensis (L.) O.Kuntze

作　　者：郭春芳[1] 唐玉海[1] 孙云[2] 陈常颂[3] 陈荣冰[3] 张木清[1] 

机构地区：[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室 [2]福建农林大学园艺学院,福州350002 [3]福建省农业科学院茶叶研究所,福建福安355015

出　　处：《热带作物学报》2008年第2期181-186,共6页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金资助项目(B0510025);福建省教育厅科技计划资助项目(JA05333)

摘　　要：利用ISSR分子标记技术,对51个茶树品种进行遗传多样性分析。选用10个多态性高、分辨力强的ISSR引物分别对供试材料的基因组DNA进行扩增,共获得53条清晰可辨的带,其中多态性带49条,多态位点百分率为92.45%,表明供试品种资源在DNA水平上存在广泛的变异。51份资源所检出的位点平均有效等位基因数、平均基因多样度、平均Shannon信息指数,分别为1.621±0.333,0.351±0.162,0.514±0.219。遗传距离介于0.097～0.692之间,平均为0.337;UPGMA法将51份资源分成4个类群。亲缘关系树状图在分子水平上显示了供试茶树品种间的亲缘关系,为今后茶树育种亲本的选配提供了理论依据。Genetic diversity of 51 tea cuhivars [Camellia sinensis （L.） O.Kuntze] was revealed by ISSR-PCR （Inter-simple sequence repeat）. A total number of 53 unambiguous bands were amplified from the 51 tea cultivars with 10 primers screened from 100 ISSR primers, 49 of which were polymorphic, and their polymorphism frequency was 92.45%. As analyzed by POPGENE 32, the average value of effective number of alleles, Nei＇s gene diversity and Shannon information index were 1.621±0.333, 0.351 ±0.162, 0.514±0.219, respectively. The Nei＇s genetic distances of the 51 tea cuhivars ranged from 0.097 to 0.692, with a mean of 0.337. The tested tea cultivars were divided into 4 groups by UPGMA （unweighted pair group method with arithmetic average） based on Nei＇s genetic distances. The genetic relationship of the 51 tea cultivars revealed by the ISSR dendrogram would be helpful for selecting the parents in tea breeding programs.

关 键 词：ISSR 茶树 遗传多样性 

分 类 号：S571.103.7[农业科学—茶叶生产加工]