紫外线与亚硝酸钠复合诱变选育L-组氨酸产生菌  

Breeding of L-Histidine Producing Bacterium by Composite Mutation with UV and NaNO_2

作　　者：梁亮[1] 邱雁临[1] 许进涛[1] 

机构地区：[1]湖北工业大学生物工程学院工业微生物湖北省重点实验室,湖北武汉430068

出　　处：《微生物学杂志》2008年第2期27-29,共3页Journal of Microbiology

基　　金：湖北省重点实验室开放基金资助项目(工大科[2004]4号文)

摘　　要：以1株谷氨酸棒杆菌(Corynebacterium glutamicum)S_6作为出发菌株,利用亚硝酸钠(NaNO_2)、紫外线(UV)进行诱变,通过实验证明亚硝酸钠诱变时间在180 s后致死率达到80%,紫外线在照射30 s后致死率达到80%。诱变后的突变菌株经6-巯基嘌呤结构类似物的抗性平板筛选,最终筛得3株菌,Y_1产L-组氨酸量达到331 mg/L,比出发菌株S_6高了5.08%,Z_2产L-组氨酸量达到325 mg/L,比出发菌株S_6高了1.9%,F_6产L-组氨酸量达到330 mg/L,比出发菌株S_6高了7.14%。结果显示,经紫外线与亚硝酸钠复合诱变后的菌株F_6产L-组氨酸的产量最高,比亚硝酸钠诱变后的菌株产L-组氨酸量提高2.06%,比紫外线诱变后的菌株产L-组氨酸量提高5.24%。Corynebacterium glutamicum S6 was taken as starting strain and mutated by NaNO2 and UV for breeding L- histidine producer. The lethality of the strain up to 80% was proved through experiment 180 s after mutated with NaNO2 , and up to 80% 30 s after UV radiation. After the mutation, the mutated strain was screened on 6-mercaptopu- rine （6-MP） analogue resistance plate and consequently got three mutant strains, the L-histidine output of Y1 was 331 rag/L, 5.08% higher than S6 ; that of Z2 was 325 mg/L, 1.9% higher than S6 ; and that of F6 was 330 mg/L, 7.14% higher than S6. The results showed that after composite mutation with UV and NaNO2 the output of L-histidine of strain F6 was the highest,2.06% higher than strain mutated with NaNO,, and 5.24% higher than strain mutated with UV.

关 键 词：L-组氨酸 紫外线 亚硝酸钠 复合诱变 

分 类 号：Q939.97[生物学—微生物学]