一种ε-聚赖氨酸降解酶活性测定的新方法  

A New Method for Activity Assay of ε-poly-L-Lys-degrading Enzyme

作　　者：赵颖[1] 贾士儒[1] 谭之磊[1] 袁国栋[1] 

机构地区：[1]天津科技大学,天津市工业微生物重点实验室,天津300457

出　　处：《食品与发酵工业》2008年第4期125-127,130,共4页Food and Fermentation Industries

基　　金：国家863项目(2006AA102347);国家“十五”科技攻关项目(2004BA713805-061);天津科技大学科研基金资助项目(20060207)

摘　　要：针对现有ε-聚赖氨酸降解酶活力测定中存在的问题。建立了一种利用甲基橙法测定ε-聚赖氨酸量的变化,从而测定ε-聚赖氨酸降解酶酶活的新方法。通过对反应温度、时间、底物浓度和缓冲体系的研究.确定了新方法的酶活测定条件。验证实验表明,新方法有较好的精确性和准确性,为ε-聚赖氨酸降解酶的研究提供了一种安全简便的测定方法。A new method that is based on the methyl orange （MeO） method for measuring the amount of ε-poly-L-Lys has been developed to present the activities of ε-poly-L-Lys-degrading enzyme. Experiments were conducted to determine the optimal reaction conditions such as temperature, incubation time, substrate concentration and buffer system. The result measured by the new method was proved to be precise. This study provided a safe and convenient method for research of ε-poly-L-Lys-degrading enzyme.

关 键 词：ε-聚赖氨酸降解酶 酶活 甲基橙法 

分 类 号：TS201.25[轻工技术与工程—食品科学]