口腔白斑、赤斑及鳞癌9p、3p的LOH/MSI及其与细胞增殖关系的研究  

Study on the relation of the LOH、MSI on chromosome 9p、3p in OLK、EK and OSCC with cell proliferation.

作　　者：王文梅[1] 段宁[1] 王亚平[2] 林昱[3] 刘晓蓉[1] 唐巍[1] 蒋文晖[1] 黄晓峰[1] 黄政[1] 

机构地区：[1]南京大学医学院附属口腔医院,江苏南京210008 [2]南京大学医学院 [3]福建医科大学

出　　处：《临床口腔医学杂志》2008年第5期303-307,共5页Journal of Clinical Stomatology

基　　金：南京市科技发展计划项目资助(20022041)

摘　　要：目的:探讨口腔黏膜癌前病变口腔白斑(OLK)、赤斑(EK)及鳞癌(OSCC)组织中染色体9p上D9S171、D9S1752、D9S1748、IFNA及3p上D3S1266、D3S643、D3S966的LOH、MSI及其与细胞增殖关系的研究。方法:应用聚合酶链反应-变性聚丙烯酰胺凝胶电泳-银染方法,检测OLK、EK及OSCC染色体9p上D9S171、D9S1752、D9S1748、IFNA及3P上D3S1266、D3S643、D3S966的微卫星位点的LOH及MSI,将检测结果与细胞增殖水平进行相关分析。结果:口腔癌前病变及鳞癌组织中9p、3p上7个位点均出现LOH和/或MSI。不同病理组别单个位点的LOH/MSI检出率无显著性差异(p>0.05)。但综合9p上4个位点或3p上3个位点或9p+3p上7个位点微卫星改变发生率,发现:9p,3p,9p+3p上LOH及LOH+MSI在不同病理组间差异显著。LOH,MSI改变状况与增殖水平关系密切。上皮异常增生程度加重,细胞增殖水平增高,LOH/MSI检出率增加。具体表现为9p、3p的LOH检出率与AgNOR计数呈正相关,MSI检出率与PCNA表达水平呈正相关。结论:9p和3p区域的基因异常是OSCC发生和发展过程高频分子事件,该区域可能存在抑癌基因。9p和3p上的微卫星改变状况在口腔癌的发生、发展中扮演重要角色,作用机制可能与其促进细胞增殖活性,使细胞无限增殖有关。Objective： To detect the LOH and MSI of microsatellite loci at chromosome 9p. 3p and their relationship with cell proliferation in OLK.EK and OSCC. Method： 83 samples were collected,including 11 cases of simple hyperplasia （HP）, 9 cases of mild dysplasia （MID）, 11 cases of moderate dysplasia （MoD）, 8cases of severe dysplasia （SD）, 12 cases of oral squamous cell carcinoma （OSCC）. A pair of genemic DNA were isolated from resected tissues and peripheral blood from each patient. PCR-PAUGE-silver staining was used to detect 7 microsatellite loci-DgS171、DgS1752、DgS1748、IFNA、 D3S1266、D3S643 and D3S966. Result：Significant differences was found between total LOH in chromosome 9p or 3p or 9p＋3p and pathological grades （P〈0.05）, so as LOH＋MSI and pathological grades. LOH has positive correlation with AgNOR number, so as MSI with PCNA index. ConcIusion：There may be some tumor suppressor gene which was related to the genesis and progression of oral squamous ceil carcinomaon chromosome 9p and 3p. LOH and MSI may play a important role in the process of oral carcinogenesis.

关 键 词：口腔 白斑 赤斑 鳞癌 杂合性缺失 微卫星 

分 类 号：R781.5[医药卫生—口腔医学]