丙烯腈对大鼠脑组织和神经胶质细胞中核因子－κB信号传导通路活性及相关基因表达的影响  

Effects of acrylonitrile on the activation of nuclear factor-κB signaling pathways and related gene expression in rat brain and rat glial cells

作　　者：焦振泉[1] 裴晓燕[2] 郭云昌[2] 徐勇[3] 

机构地区：[1]中国疾病预防控制中心公共卫生管理处,北京100050 [2]中国疾病预防控制中心营养与食品安全所 [3]美国印第安那大学医学院

出　　处：《卫生研究》2008年第3期264-268,共5页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30400357)

摘　　要：目的丙烯腈(ACN)对大鼠脑组织和大鼠神经胶质细胞中核因子κB(NF-κB)信号传导通路活性及相关基因表达的影响。方法利用micorarray和EMSA实验测定了SD大鼠经0和200mg/kg ACN分别作用14、28和90天后,大鼠脑组织NF-κB信号传导通路活性的变化及相关基因表达的变化;大鼠神经胶质细胞经0、25、50和75μg/ml ACN作用4和24h后细胞NF-κB信号传导通路活性的变化及相关基因表达的变化。结果ACN促进大鼠脑组织(体内)和神经胶质细胞(体外)NF-κB信号传导通路相关基因(NIK、IKK、NF-κB1、NF-κB2、IκBa和c-myc)的表达。EMSA实验也进一步证实了ACN可激活大鼠脑组织和大鼠神经胶质细胞中NF-κB信号传导通路。结论ACN诱导的氧化应激和ACN激活NF-κB信号传导通路具有一定的关系。Objective To study the effects of acrylonitrile on the activation of nuclear factor κB（NF-κB） signaling pathways and related gene expressions in rat brain and rat glial cells. Methods The microarray and EMSA （electrophoretic mobility shift assay）techniques were used to investigated NF-κB pathway and related gene expressions changes in rat brain after treated with 0 mg/kg and 200mg/kg ACN fur 14,28 and 90 days and rat normal glial cells after treated with 0, 25, 50 and 75μg/ml ACN for 4 and 24 hours. Results ACN induced the NF-κB pathway related gene expression （NIK, IKK, NF-κB1, NF-κB 2, IκBa, c-myc） in rat brain （in vivo） and in rat glial ceils （in vitro）. It was confirmed by EMSA results that ACN caused activation of the NF-κB pathway in rat brain and rat glial cells. Conclusion There is some relationship between gene expression of NF-κB pathway and ACN-induced oxidative stress.

关 键 词：丙烯腈 核因子ΚB 神经胶质细胞 

分 类 号：Q593.2[生物学—生物化学] R730.231[医药卫生—肿瘤]