把人参二醇类皂苷转化为Rg3的特种人参皂苷糖苷酶生成的细菌筛选  

Ginsenosidase transformating protopanaxadiol type saponin to gisenoside by different bacteria

作　　者：于小溪[1] 姜晓野[1] 王岩[1] 鱼红闪[1] 安东善 柳青梅 任皖泽 金凤燮[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]韩国科学技术院环境与分子微生物实验室,韩国大田305-701

出　　处：《大连工业大学学报》2008年第2期97-101,共5页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省高等学校优秀人才支持计划资助项目(RC-04-05);辽宁省科学技术基金资助项目(20042132);国家外国专家局国际合作项目(20062100374)

摘　　要：采用TLC、HPLC等酶活力检测方法对Arthrobacter sp.3、Rhodanobacter sp.14、Rhodopseudo-monas sp.18和Enterobacter sp.20菌进行了菌种筛选。其中Arthrobacter sp.3、Rhodopseudomonas sp.18和Enterobacter sp.20菌发酵生成把人参二醇类皂苷PPD转化为Rg3、Rh2和苷元的特殊人参皂苷糖苷酶,Arthrobacter sp.3菌发酵产酶速度相对快一些。Rhodanobacter sp.14发酵主要生成能把人参二醇类皂苷PPD转化为Rd的人参皂苷糖苷酶。Rhodanobacter sp.14发酵生成的人参皂苷糖苷酶对丙酮抗性较好,Arthrobacter sp.3、Rhodopseudomonas sp.18和Enterobacter sp.20菌所生成的人参皂苷糖苷酶对丙酮抗性略差。综合各因素,选取Arthrobacter sp.3为目标菌种。The ginsenosidases produced by Arthrobacter sp. 3, Rhodanobacter sp. 14, Rhodopseudomonas sp. 18 and Enterobacter sp. 20 was determined with TLC and HPLC. Arthrobacter sp. 3, Rhodopseudomonas sp. 18. and Enterobacter sp. 20 produced ginsenosidase trasformating the protopanaxadiol type saponin to ginseonoside Rg3, Rh2 and aglycone. The ginsenosidases production by Arthrobacter sp. 3. was rapider than that by others. Rhodanobacter sp. 14 produced ginsenosidase trasformating the panaxadiol type saponin to ginseonoside Rd. The ginsenosidase from Rhodanobacter sp. 14 strain was stable on acetone treatment, but the enzyme from Arthrobacter sp. 3, Rhodopseudomonas sp. 18 and Enterobacter sp. 20 strains was not stable.

关 键 词：人参皂苷 酶转化 细菌 

分 类 号：Q556.2[生物学—生物化学]