电堆集-非水毛细管电泳分离测定甘草次酸和阿魏酸的研究  

Simultaneous Separation and Determination of Glycyrrhetinic Acid and Ferulic Acid by Sample Electrostacking-Non-aqueous Capillary Electrophoresis

作　　者：李利军[1] 吴峰敏[2] 程昊[1] 喻来波[3] 冯军[3] 吴健玲[3] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]河南科技大学化工与制药学院,河南洛阳471003 [3]广西大学化学与化学工程学院,广西南宁530005

出　　处：《分析测试学报》2008年第6期611-614,618,共5页Journal of Instrumental Analysis

基　　金：广西自然科学基金资助项目(桂科自0481019);广西教育厅科学研究基金资助项目(桂教科[2000]392)

摘　　要：建立了电堆集富集-非水毛细管电泳（NACE）同时分离测定甘草中甘草酸和阿魏酸的新方法。系统研究了电压、非水溶剂、乙酸钠浓度、氢氧化钠浓度、样品溶剂等因素对电堆集和分离效果的影响，确立了分离和富集的最佳条件。以甲醇-乙腈（体积比4：6）为非水介质，30mmol·L^-1乙酸钠-2．5mmol·L^-1氢氧化钠为背景电解质；分离电压-20kV，检测波长254nm。在优化条件下，甘草次酸在0．80～12．80mg·L^-1（r=0.9996），阿魏酸在0．77～45．60mg·L^-1（r=0．9996）质量浓度范围内线性关系良好，回收率分别为97％～102％，98％～102％；甘草次酸检出限为0．069mg·L^-1，阿魏酸检出限为0．087mg·L^-1，RSD均不大于4．6％。A new method for the simultaneous separation and determination of glycyrrhetinic acid （GA） and ferulic acid（FA） in liquorice by sample electrostacking -nonaqueous capillary electrophoresis was proposed. The effects of separation voltage, non-aqueous solvent, concentrations of NaAc and NaOH, and sample solvent were studied. The operations were performed in a buffer system of NaAc （30 mmol· L^-1 ）and NaOH （2.5 mmol· L^-1 ） in methanol - acetonitrile nonaqueous medium at a separation voltage of- 20 kV and the detection wavelength was set at 254 nm. The linear range of the calibration curve for GA was 0. 80 - 12. 80 mg · L^-1 with a detection limit of 0. 069 mg · L^-1 and that for FA was 0.77 - 45.60 mg · L^-1 with a detection limit of 0. 087 mg · L^-1. The method has been applied to the determinations of GA and FA in liquorice with satisfactory results. The recovery ranges obtained for GA and FA were 97% - 102% and 98% - 102% , respectively, and the RSD was less than 4.6% .

关 键 词：电堆集 非水毛细管电泳 甘草次酸 阿魏酸 甘草 

分 类 号：O657.8[理学—分析化学] S567.71[理学—化学]