一种Vc二步发酵伴生菌的筛选方法  

A Screening Method of Associated Bacteria in Two-stage Fermentation of Vitamic C

作　　者：康艳红[1] 张海宏 赵军[1] 杨伟超[1] 孙彩霞[3] 徐慧[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]东北制药总厂,辽宁沈阳110026 [3]东北大学,辽宁沈阳110004

出　　处：《生物技术》2008年第3期42-44,共3页Biotechnology

摘　　要：采用发酵种液离心法对Vc二步发酵伴生菌筛选方法进行研究。将发酵12h的发酵液4000r/min离心10min,取上清液以20%的接种量接入种子发酵液中(30ml/250ml),再接入1/20环大菌,29℃振荡(180r/min)培养24h,将发好种液以15%接种量转接摇瓶发酵(20ml/250ml),29℃振荡(180r/min)培养,待残糖降至1mg/ml以下测酸。摇瓶发酵结果表明,此方法易操作、效率高,可应用于伴生菌筛选的初筛过程中。A new screening method of associated bacteria in two-stage fermentation of vitamin C was described in this article.The procedure is： the Suspended media cultured for 12 hours were centrifugated for 10 rain at 4 000r/rain, 6 ml of the centrate containing Gluconobacter oxydans cell suspension was inoculated into 30 ml seed culture medium in 250ml flakes, cultured for routinely（180r/min）24h at 29℃ .Thereafter,3 ml of it culture were inoculated in 20 ml fermentation culture medium in 250ml flakes, cultured mutinely（180r/min） at 29℃ the lsorhose in medium below lmg/ml. The result of experiment, proved that this method is feasible, and easy to operate, moreover, the breeding cycle was short and the efficiency is very high.

关 键 词：2-酮基-L-古龙酸 氧化葡萄糖醪杆菌 二步发酵 离心分离 

分 类 号：Q933[生物学—微生物学]