Annexin A1在小鼠胚泡植入不同时期子宫内膜组织中的表达及意义  

Expression of Annexin A1 in Endometrium during the Blastocyst Implantation in Mouse

作　　者：张群[1] 刘学庆[1] 陈雪梅[1] 王应雄[1] 赖雪梅[1] 何俊琳[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生物学研究室

出　　处：《生殖与避孕》2008年第5期257-263,共7页Reproduction and Contraception

基　　金：国家自然科学基金项目(No.30500054);重庆市科委重点项目(No.2004-47)

摘　　要：目的:探讨Annexin A1在小鼠胚泡植入期的作用。方法:双向聚丙烯酰胺凝胶电泳(2D-PAGE)分析孕d3、d5、d7小鼠子宫内膜蛋白质组,用差异蛋白质组学显示pI约5.1、分子量约38kD的蛋白点在d3、d5上调,且在d5更明显。经基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)测定其胶内酶解后的肽质量指纹谱(peptide mass fingerprint,PMF)和数据库搜索对此蛋白点进行鉴定;再用RT-PCR、原位杂交、Western blot和免疫组织化学技术进行验证分析。结果:该蛋白点鉴定为鼠源性Annexin A1;d5小鼠子宫内膜组织中Annexin A1的mRNA和蛋白质水平均增加。结论:Annexin A1可能参与胚泡着床这一重要生命活动过程。Objective： To investigate the role of Annexin A1 during the blastocyst implantation of mouse. Methods： Total proteins of mice endometria from pregnant d 3, d 5 and d 7 were analyzed by two- dimensional gel electrophoresis and image analysis, respectively. Differential expression protein spots were excised by dot-gel digestion with trypsin. Then peptide mass fingerprinting （PMF） was submitted to an appropriate software （Mascot： PMF） for identifying proteins. A differential protein spot was checked by RT-PCR, Western blotting, immunohistochemistry and in-situ hybridization. Results： The differential protein spot was identified as Annexin A1 by PMF. The expression level of Annexin A1 mRNA and protein reached the maximum at the d 5 of pregnant. Conclusion： Annexin A1 might play an important role during the blastocyst implantation.

关 键 词：ANNEXIN A1 胚泡植入 蛋白质组 小鼠 子宫内膜 

分 类 号：R-33[医药卫生]