利用制胶废水生产5-氨基乙酰丙酸的发酵条件研究  

Study on the fermentation conditions of 5-aminolevulinic acid produced with rubber wastewater

作　　者：朱春节[1] 谢秀祯[1] 王小明[1] 黄循吟[1] 覃绪林[1] 

机构地区：[1]海南师范大学生物系,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2008年第2期184-188,共5页Journal of Hainan Normal University（Natural Science）

基　　金：海南省教育厅高校科研基金项目(Hjkj200412)

摘　　要：利用制胶废水作为营养物对类球形红细菌突变株A5产ALA的发酵条件进行了初步优化,结果表明在体积分数为80%制胶废水中添加终质量浓度为4 g/L的废糖蜜作碳源,接种量为10%,装液量为20 mL/100mL,在180 r/min黑暗振荡培养24 h后加入终浓度为20 mmol/L的乙酰丙酸,再于100 r/min继续振荡培养24 h,ALA产量可达8.45 mg/L.By using rubber wastewater as basic nutrients, a preliminary optimization of fermentation conditions for the mutant strain A5 of rhodobacter sphaeroides was conducted to produce ALA in Shake bottle fermentation. The results showed that under the fermentation condition in which 0.4% molasses was supplemented in 80% rubber wastewater as carbon source, inoculum size was 15%, 20mL medium was charged in a 100mL flask, 20mmol/L of levulinic acid was added into the medium after cultivation at 180r/min in darkness for 24h, and then next 24h cultivation continued at 100r/min, the output of ALA reached up to 8.45mg/L.

关 键 词：5-氨基乙酰丙酸 类球形红细菌 制胶废水 发酵条件 

分 类 号：TQ921[轻工技术与工程—发酵工程]