交联壳聚糖固定α-淀粉酶的研究  

Research on the Immobilization of α-amylase with Chitosan Glutaraldehyde

作　　者：蒋珍菊[1] 朱亮[1] 林国秀[1] 刘洪[1] 

机构地区：[1]西华大学生物工程学院,四川成都610039

出　　处：《西华大学学报（自然科学版）》2008年第4期88-90,共3页Journal of Xihua University:Natural Science Edition

基　　金："西华杯"学生课外科技学术科技作品项目资助(No.2008177)

摘　　要：选择了具有生物活性的壳聚糖为载体、戊二醛为交联剂来固定化α-淀粉酶,探索了固定化的最佳条件。结果表明:最佳固载量为每克壳聚糖50mgα-淀粉酶,交联剂戊二醛浓度为15%,处理温度均为室温,处理时间以5h为宜,固定化pH值以7较好。交联壳聚糖用于固定α-淀粉酶行之有效,且效率较高,其酶活回收率可达82.2%。On the condition of cross link by glutaraldehyde,the chitosan was chosen as the carrier of α-amylase due to its biocompatibility.The condition of the immobilization of α-amylase was studied.The optimum amount of enzyme immobilized on chitosan was 50mg/g chitosan.The optimum temperature and time of glutaraldehyde reacting with chitosan were ambient temparature and 5 hour respectively.The concentraion of the glutaraldehyde was 15%.The optimum pH value of the immobilized enzyme was 7.0 for α-amylase.This research certified that it was practicable and efficient that α-amylase was immobilized on chitosan with glutaraldehyde.The recovery of immobilized enzymes activity was 82.2%.

关 键 词：壳聚糖 戊二醛 固定化酶 Α-淀粉酶 

分 类 号：O636.1[理学—高分子化学]