羊驼性别决定基因sry部分片段的克隆与序列分析  

Cloning and Sequence Analysis of the Partial Sequence of Sex Determination Gene sry in Alpaca(Lama pacos)

作　　者：贺俊平[1] 董常生[1] 杨勇[1] 游蓉丽[1] 高莉[1] 王海东[1] 谢建山[1] 

机构地区：[1]山西农业大学动物科技学院,山西太谷030801

出　　处：《激光生物学报》2008年第3期363-366,共4页Acta Laser Biology Sinica

基　　金：国际先进农业科学技术"948"项目(2003-Z86)

摘　　要：从成年羊驼血液提取基因组DNA,参照哺乳动物sry(sex-determining region on the Y chromosome)基因的同源保守区域设计特异性引物,用PCR技术成功扩增羊驼sry基因的部分片段,且全部实验雄性个体均成功扩增,而雌性个体则无任何特异性片段,说明所扩增基因片段具雄性特异性。对扩增序列所编码蛋白序列分析显示所扩增的片段编码的蛋白序列在哺乳动物sryHMG-box(high mobility group box)蛋白超家族的HMG-box区域,说明扩增的为sry基因片段。用所扩增片段与其他哺乳动物同源序列分析显示,由sry基因构建的系统进化树,与传统的动物分类关系相近,说明由sry基因的HMG-box构建系统树是分析物种亲缘关系的有效工具。Partial sequence of alpaca sry gene was cloned from alpaca genome DNA by PCR technique. The amplification results indicated that the sequence was only found in male alpaca. The peptide coded by the partial sequence was in the HMG-box of mammallian sry HMG-box superfamily, which suggested that the sequence was a partial of sry gene. According to the sry homology sequence among alpaca and others mammals we constructed the phylogenetic tree. The evolutionary relationship was similar with traditional view. The sry phylogenetic tree was an effective tool for inferring the relationship of mammals.

关 键 词：羊驼 性别决定 SRY基因 

分 类 号：S823.3[农业科学—畜牧学]