光动力对细菌的杀伤作用研究  

Photodynamic inactivation of bacterial

作　　者：任雅清[1] 唐书泽[1] 吴希阳[1] 毕水莲[1] 陈振强[2] 

机构地区：[1]暨南大学食品科学系,广州510632 [2]暨南大学光电工程系,广州510632

出　　处：《中国调味品》2008年第6期37-40,共4页China Condiment

基　　金：粤港招标项目:农产品深加工技术及安全(2005A20303002)

摘　　要：通过细菌的平板菌落计数实验,研究了血啉甲醚(Hematoporyrin monomethyl Ether,HMME)对革兰氏阳性菌蜡样芽胞杆菌和革兰氏阴性菌大肠杆菌的光动力杀伤作用。结果表明:当HMME浓度为5mg/mL,可见光光照30min时96.3%的蜡样芽胞杆菌被杀死,相同浓度时,光照的杀灭效果强比不光照强;当HMME浓度为150 mg/mL,可见光光照30min时99.9%的大肠杆菌杆菌被杀死,不光照,HMME对大肠杆菌基本上没有杀伤作用。因此,在可见光光照条件下,微量的HMME对革兰氏阳性菌有很强的杀菌效果,微量的HMME对革兰氏阴性菌的杀菌作用不明显,高剂量时则对其杀菌效果理想。The photodynamic inactivation of Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus cereus by hematoporyrin monomethyl ether were investegated through the reduction of colony unit. Results showed that 96.3% of Bacillus cereus were photoinactivated by illumination with 30 min visible light in the presence of 5 mg/mL HMME. The cell killing effect to Bacillus cereus with illumination was more obvious than in the dark at the same concentration of HMME; 99.9% of E. coli were photoinactivated by il- lumination with 30 min visible light in the presence of 150 mg/mL HMME, but the killing effect was not significant to E. coli in the dark. The Gram-positive bacteria can be effectly photoinactivated by HMME, Gram-negative bacteria were susceptible to photosensitized destruction with high concetration of HMME.

关 键 词：血啉甲醚 大肠杆菌 蜡样芽胞杆菌 光动力杀伤 

分 类 号：TS201.3[轻工技术与工程—食品科学]