双酚A完全抗原的制备  

Study on Preparation of Complete Antigen of Bisphenol A

作　　者：石春红[1] 章银珠[1] 俞晔 孙秀兰[1] 姚卫蓉[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]张家港检验检疫局,江苏张家港215633

出　　处：《食品与生物技术学报》2008年第3期37-40,共4页Journal of Food Science and Biotechnology

基　　金：国家质检总局科技计划项目(2007IK271)

摘　　要：采用重氮化法在双酚A(BPA)上引入羧基,将其与牛血清白蛋白(BSA)偶联,制备双酚A完全抗原BPAH-BSA。紫外扫描光谱和红外光谱实验表明偶联成功,为进一步制备抗BPA抗体提供了良好的免疫原。Bisphenol A hapten （BPAH） was prepared by diazotization, and then coupled with carrier protein bovine serum albumin （BSA） to prepare complete antigen BPAH--BSA. Both UV scanning and IR spectrum indicated that the complete antigen BPAH-BSA was successfully synthesized. The result present here demonstrated that an excellent immune antigen for further preparation of BPA antibody was achieved.

关 键 词：双酚A 完全抗原 牛血清白蛋白 紫外扫描光谱 红外光谱 

分 类 号：TS207.3[轻工技术与工程—食品科学]