前体物质与真菌激发子对虫草菌素产量的影响  

Effect of Precursor and Fungal Elicitor on Cordycepin Production

作　　者：李祝[1] 郁建平[2] 梁宗琦[1] 肖洋 

机构地区：[1]贵州大学生命科学院真菌资源研究所,贵州贵阳550025 [2]贵州大学生化营养研究所,贵州贵阳550025 [3]贵州省产品质量检验检测院,贵州贵阳550003

出　　处：《食品科学》2008年第6期273-275,共3页Food Science

基　　金：贵州省科技厅科学技术基金项目[(2007)2037]

摘　　要：将腺苷作为前体,疫霉Phytophthora sp.(YL)作为真菌激发子,研究它们对蛹虫草(Cordyceps millitaris97-0706)菌株虫草菌素产量及生物量的影响。结果表明:腺苷以3.6mg/ml的量在发酵前(0h)单独加入,摇瓶培养7d,0706菌株的虫草菌素含量达到了8.55±0.09mg/g(p<0.01),是对照的2.2倍。当2.0mg/ml腺苷与80μg/mlYL混合加入,0706菌株的虫草菌素含量为8.25±0.08mg/g,与只用2.0mg/ml腺苷处理的含量(8.21±0.074mg/g)无显著差异(p>0.05),仅表现了腺苷的作用;生物量方面,腺苷和YL的同时加入,起到了协同提高的作用,0706菌株的生物量最终达到46.11±0.10g/L(p<0.01),是对照的1.9倍。Effects of adenosine and Phytophthora sp. （YL） on the cordycepin production and biomass of Cordyceps millitaris 97-0706 were studied. The ruselts showed that 3.6 mg/ml adenosine added into shake flask culture before fermentation, after 7 d the content of cordycepin reaches to 8.55 ± 0.09 mg/g （p 〈 0.01 ）, 2.2 times more than that of control; 2.0 mg/ml adnosine and 80 μ g/ml YL together added into shake flask at starting, the cordycepin production of 0706 reaches to 8.25 ± 0.08 mg/g, which is no significant difference （p 〉 0.05） to cordycepin production with only 2.0 mg/ml adenosine added （8.21 ±0.074 mg/g）. But adenosine and YL could cooperate to enhance the mycelia biomass of 0706 （46.11± 0.10 g/L）, 1.9 times of that of control.

关 键 词：腺苷 虫草菌素 激发子 蛹虫草 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]