微乳液介质中巯基葡聚糖凝胶分离富集催化动力学光度法测定痕量硒(IV)(英文)  

Catalytic Kinetic Spectrophotometric Determination of Trace Selenium (IV) in Microemulsion after Separation and Enrichment by SDG

作　　者：周之荣[1] 王群[2] 章淑媛[2] 张纪文 张圆玲 苏志文 

机构地区：[1]广东药学院公共卫生学院,广东广州510240 [2]东华理工大学校医院,江西抚州344000 [3]张家港市质量技术监督局,江苏张家港224008 [4]江西省临川第一中学,江西抚州344100

出　　处：《食品科学》2008年第6期292-297,共6页Food Science

基　　金：广东药学院科研基金资助项目(2007GGW03)

摘　　要：基于弱酸介质中,在微乳液介质存在时,痕量硒(IV)催化KBrO3氧化2-(5-硝苯2-吡啶偶氮)-5-二已氨基酚(5-NO2-PADAP)的褪色反应,建立了测定痕量硒(IV)的动力学光度法。在固定加热时间段(8min)后,于450nm处测定5-NO2-PADAP的吸光度降低值监控反应速率。方法检出限为0.013μg/L,校准曲线的浓度线性范围为0.0～1.0μg/L。结合巯基葡聚糖凝胶分离富集,方法用于食物和人发样品中痕量硒(IV)的测定,结果与ICP-AES测定值相符,相对标准偏差为2.9%～4.3%,加标回收率为96.3%～103.8%。A catalytic spectrophotometry for the determination of trace selenium （IV） based on its catalytic effect on discoloring reaction of oxidation of with potassium bromate in weak acid solution and in the presence of microemulsion medium is described. The above reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 450 nm for the catalytic reaction of 5-NO2-PADAP. Under optimum conditions, the calibration graph is linear in the range of 0.0- 1.0 μg/L of Se （IV） at 450 nm. The detection limit achieved is 0.013 μ g/L. Combined with sulphydryl dextrane gel （SDG） separation and enrichment, the method has been successfully applied to the determination of Se （IV） in foodstuff and human hair samples with the relative standard deviation of 2.9 % ~4.3 % and the recovery of 96.3 % ~ 103.8 %, the results are in good agreement with those provided by ICP-AES method.

关 键 词：催化动力学光度法 硒(IV) 2-(5-硝苯2-吡啶偶氮)-5-二已氨基酚(5-NO2-PADAP) 微乳液 分离 富集 

分 类 号：TS201.26[轻工技术与工程—食品科学] O657.32[轻工技术与工程—食品科学与工程]