分光光度法测定山茱萸及其保健酒中三萜酸的含量  

Determination of Tritepenoidic Acid in Cornus officinalis and Its Health Wine by Spectrophotometry

作　　者：杨剑芳[1] 路福平[1] 黄明勇[2] 高文远[3] 杜连祥[1] 

机构地区：[1]天津工业微生物重点实验室,天津科技大学生物工程学院,天津300457 [2]泰达生态园林发展有限公司,天津300457 [3]天津大学药物科学与技术学院,天津300072

出　　处：《食品科学》2008年第6期321-323,共3页Food Science

摘　　要：以熊果酸为标准品,5%香草醛-冰醋酸、高氯酸为显色剂,用紫外-可见分光光度法在538nm处测定山茱萸及其保健酒中三萜酸的含量。结果表明,熊果酸的线性范围为26.4～158.4μg,回归方程为A=0.0071C-0.0941(R2=0.9994),平均回收率为98.94%,RSD为2.31%(n=5);山茱萸及其保健酒中三萜酸含量分别为2.511%、2.091%。该方法简便、快捷、重复性好,适合于山茱萸及其保健酒中三萜酸的含量测定。Based on ursolic acid as reference standard and 5% vanillirt-acetum and perchlofic acid as color developer, the contents of tritepenoidic acid in Comus officinalis and its health wine were determined by spectrophotometry at 538 nm. The results showed that in the range of 26.4 to 158.4pg for ursolic acid, the linear correlation equation is A=0.0071C-0.0941 （R 2=0.9994） and the average recovery rate is 98.94%, with RSD2.31% （n=5）; The contents of tritepenoidic acid in Comus officinalis and its health wine are 2.511% and 2.091% respectively. This method is fast, accurate, reliable and suitable for determination of tritepenoidic acid.

关 键 词：山茱萸 山茱萸保健酒 三萜酸 紫外-可见分光光度法 

分 类 号：TS207.3[轻工技术与工程—食品科学]