血啉甲醚对蜡样芽胞杆菌和大肠杆菌的光动力杀伤作用  

PHOTODYNAMIC INACTIVATION OF BACTERIAL BY HEMATOPORYRIN MONOMETHYL ETHER

作　　者：任雅清[1] 唐书泽[1] 吴希阳[1] 毕水莲[1] 陈振强[2] 

机构地区：[1]暨南大学食品科学系,广东广州510632 [2]暨南大学光电工程系,广东广州510632

出　　处：《食品研究与开发》2008年第7期140-144,共5页Food Research and Development

基　　金：粤港招标项目:农产品深加工技术及食品安全(2005A20303002)

摘　　要：通过细菌的平板菌落计数和生长延滞实验,研究了血啉甲醚(Hematoporyrin monomethyl Ether,HMME)对革兰氏阳性菌蜡样芽胞杆菌和革兰氏阴性菌大肠杆菌的光动力杀伤作用。结果表明:当HMME浓度为5mg/L,可见光光照30min时90%的蜡样芽胞杆菌被杀死,其生长曲线明显受到延滞。相同浓度时,光照的杀灭效果比不光照强;但同等条件下无论是光照还是不光照,对大肠杆菌未见显著杀伤效果。因此,在可见光光照条件下,HMME对革兰氏阳性菌有较好的杀菌效果,对革兰氏阴性菌作用不明显。The photodynamic inactivation of Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus cereus by hematoporyrin monomethyl ether were investegated through the reduction of colony unit and bacteria inactivation rate. Results showed that 90 % of Bacillus cereus were photoinactivated and the growth was delayed by illumination with 30 min visible light in the presence of 5 mg/L HMME. The cell killing effect to Bacillus cereus with illumination was more obvious than in the dark at the same concentration of HMME, but it was not significant to E. coli no matter with illumination or in the dark. The Gram-positive bacteria can be photoinactivated by HMME, but Gram-negative bacteria were not susceptible to photosensitized destruction.

关 键 词：血啉甲醚 大肠杆菌 蜡样芽胞杆菌 光动力杀伤 

分 类 号：R155.3[医药卫生—营养与食品卫生学]