低温α-淀粉酶产生菌GS230发酵条件与酶学性质研究  

Enzyme characteristics and production conditions of cold-active α-amylase producd by Pseudoalteromonas sp.GS230

作　　者：刘红飞[1] 吕明生[2] 王淑军[2] 房耀维[2] 李华钟[1] 

机构地区：[1]江南大学教育部工业生物技术重点实验室,江苏无锡214122 [2]淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港222005

出　　处：《中国酿造》2008年第7期13-18,共6页China Brewing

基　　金：国家自然科学基金项目(40746030)

摘　　要：对分离自连云港海域的低温α-淀粉酶产生菌Pseudoalteromonas sp.GS230进行了产酶条件的优化和酶学性质的研究。结果表明,该酶在0.4%可溶性淀粉、0.25%牛肉膏、1.5%蛋白胨以及5%接种量、培养32 h产酶量达到最高。对低温α-淀粉酶性质进行研究发现,该酶的最适作用温度和pH值分别是30℃和pH 8.0、0℃时相对酶活达28%。对酶稳定性的研究发现,该酶对热敏感,30℃半衰期为1.5h,Ca2+可以提高酶的稳定性;在反应体系中添加5 mmol/L Ca2+,30℃保温1.5h,残余酶活达93%。Na+、K+、Ca2+等对酶有激活作用,而Pb2+、Cu2+、Fe3+等强烈抑制酶活性。Enzyme characteristics and production condition of cold-active a-amylase produced by Pseudoalteromonas sp. GS230, which was isolated from Lianyungang seaside, were studied. The maximum activity was recorded at 32 h when cultured in 0.4% soluble starch, 0.25% beef extract and 1.5% peptone with 5% inoculation amount. The optimal temperature and pH of a-amylase production were 30℃ and pH 8.0 respectively. The enzyme was sensitive to heat. The half life of the enzyme was 1.5 h at 30℃. Ca^2＋ could enhance thermostability of the enzyme. When 5 mmol/L Ca^2＋ was added, the residual activity of the amylase was 93% after being kept at 30℃ for 1.5 h. The activity of α-amylase was stimulated by Na^＋, K^＋ and Ca^2＋, but intensely inhibited by Fe^3＋, Pb^2＋ and Cu^2＋.

关 键 词：低温α-淀粉酶产生菌 低温α-淀粉酶 产酶条件 酶学性质 

分 类 号：Q935[生物学—微生物学]