透明质酸和泼尼松龙对间接性颞下颌关节损伤治疗效果的实验研究  

Experimental study of hyaluronate and prednisolone on indirect temporomandibular joint trauma

作　　者：张旭东[1] 董福生[1] 任贵云[1] 白九评[1] 陈瑞雪[1] 

机构地区：[1]河北医科大学口腔医学院,石家庄050017

出　　处：《现代口腔医学杂志》2008年第3期296-299,共4页Journal of Modern Stomatology

基　　金：河北省科技厅资助课题(072761670)

摘　　要：目的探讨透明质酸和泼尼松龙对间接性颞下颌关节损伤的治疗作用。方法选用新西兰白兔36只,分为3个实验组:透明质酸治疗组、泼尼松龙治疗组、损伤对照组和1个正常对照组。切除标本通过光镜,扫描电镜,组织化学方法观察。结果损伤后3天泼尼松龙组显示对损伤反应明显的抑制作用,7天、30天、90天时,HA组促进损伤修复的作用显著,髁突前斜面增殖带PCNA阳性细胞计数较泼尼松龙组多(P<0.01)。结论HA在TMJ损伤早期对损伤的抑制作用小于泼尼松龙。HA对促进TMJ间接性损伤后的修复作用优于泼尼松龙。Objective To study the effects of hyalurate and prednisolone on temporomandibular joint （TMJ） trauma by established animal models with the indirect TMJ trauma. Methods Thirty - six New Zealand white rabbits were beaten at the mandibular body by a 4kg hammer to establish the animal models of indirect TMJ trauma. The animal were then randomly and equally divided into 3 groups, for injection with hyaluronate （group HA） and prednisolone （group PL） at the bilateral TMJ, and 0.9% saline only at the left TMJ （group SC）, respectively. All the animals were sacrificed respectively at day 3, 7, 30, and 90 after trauma. Another 2 rabbits without trauma were sacrificed as the normal control. All specimens were observed under light microscope （LM） and were stained with immunohistechemistry against PCNA. The specimens of condyloid process were observed under scanning electron microscope （SEM）. Results The extent of trauma in group PL was lighter than that in group HA at day 3 after trauma. At day 7, 30 and 90, the number of positive PCNA cartilage cells in HA were more than those in group PL （ P 〈 0.01 ）. Conclusion Inhibition of Hyaluronic acid on TMJ trauma in a short period was less than that of Prednisolone. Hyaluronic acid was more effective than Prednisolone in treating indirect TMJ trauma.

关 键 词：动物模型 颞下颌关节 透明质酸 泼尼松龙 间接性损伤 

分 类 号：R782[医药卫生—口腔医学]