酸应激对1株乳酸乳球菌质膜F_1F_0-ATPase活性的影响  

Influence of Acid Tolerance Response on Membrane F_1F_0-ATPase Activity in Lactococcus lactis

作　　者：刘怀龙[1] 孟祥晨[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室/食品学院

出　　处：《食品与发酵工业》2008年第5期63-65,共3页Food and Fermentation Industries

基　　金：东北农业大学创新团队资助项目

摘　　要：评价10株乳酸乳球菌的酸应激能力,选用应激能力较高的菌株,确定其最佳酸应激条件。将4 h、10 h、20 h培养的该菌菌体在此条件下应激,以硫酸亚铁钼蓝比色法测定应激前后的质膜F_1 F_0-ATPase活性。结果表明,菌株KLDS 4.0312具有较高应激能力,应激后耐酸性可提高7542倍,其最佳酸应激条件为pH4.5,30 min。4 h、10 h、20 h培养的该菌菌体应激后,质膜F_1F_0-ATPase活性分别提高56.68%、11.05%、5.16%。Acid Tolerance Response （ATR） effects of 10 Lactococcus lactis strains were evaluated, the strain which has the best ATR effect was used in this research, and the optimal conditions for ATR were set out. Under the optimal condition for ATR, the membrane FIF0-ATPase activity of adapted cells and non-adapted cells was assessed by measuring inorganic phosphate released from ATP hydrolysis. Results KLDS4. 0312 has the best ATR effect and the optimal conditions for ATR were pH 4.5,30 min. The acid tolerance of KLDS4.0312 was increased by 7542 times after acid adaptation. Membrane F1F0-ATPase activity after optimal ATR was increased by 56.68%, 11.05% and 5.16% when the bacteria were incubated for 4h, 10h and 20h, respectively.

关 键 词：酸应激 乳酸乳球菌 F1F0-ATPase 存活率 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]