羟乙基纤维素及蔗糖无胶筛分毛细管电泳分离DNA片段  

Separation of DNA Fragments by Capillary Electrophoresis with Non-gel Sieving Matrix of Hydroxyethyl Cellulose and Sucrose

作　　者：陈洪[1] 宋立国[1] 熊少祥[1] 程介克[1] 

机构地区：[1]武汉大学化学系,武汉430072

出　　处：《高等学校化学学报》1997年第11期1769-1773,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：提出以羟乙基纤维素、蔗和硼酸形成"互穿网络"无胶筛分介质，显著地提高了分离效率．分离了ΦX174／HaeⅢDNA的所有11个DNA片段．探讨了"互穿网络"无胶筛分机理．将该方法应用于决定鳝鱼性别基因PCR扩增产物的分离与鉴定，结果较好．We demonstrate that hydroxyethyl cellulose, sucrose and boric acid can formnongel sieving matrix with interpenetrating polymer networks (INPs). This kind of INPs mixednon-gel sieving matrix can greatly improve DNA separation efficiency. While all elevenfragments of φX174/Hae Ⅲ DNA can not be completely separated with a sieving matrix of 0. 8%hydroxyethyl cellulose in 1×TBE buffer, they are successfully separated by a non-gelsieving matrix of 0.8% hydroxyethyl cellulose, 10% sucrose in 1×TBE buffer. The reactionmechnism to form the INPs has been proposed. The length of a PCR magnified DNAfragment from sex-related gene in rice-field cells is also determined, which is in good agreementwith slab gel electrophoresis.

关 键 词：无胶筛分 毛细管电泳 DNA片段 性别决定 分离 

分 类 号：Q523.03[生物学—生物化学] Q781