壳聚糖对背根神经节细胞的促黏附作用  

Chitosan coat promotes the cell growth of the dosal root ganglon

作　　者：陈益人[1] 陈罡[1] 倪温慨[1] 杨宇民[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室,226001

出　　处：《江苏医药》2008年第7期706-708,共3页Jiangsu Medical Journal

基　　金：南通大学自然科学基金项目(05Z112)

摘　　要：目的探讨壳聚糖对背根神经节(DRG)细胞生长的影响。方法用多聚赖氨酸与壳聚糖做DRG细胞培养涂料,观察在两种条件下DRG的生长情况;并以实时定量PCR检测两组细胞A4galT基因的表达量。结果壳聚糖对DRG细胞有促黏附作用,可引起DRG细胞A4galT基因表达上调。结论壳聚糖对DRG细胞生长具有良好的生物相溶性,可以用作实验室良好而且廉价的培养皿涂料。Objective To study the effect of chitosan on the cell growth of the dosal root ganglon（DRG）. Methods DRG cells were cultured with poly-D-lysine or chitosan-coated culture dish. A4galT gene expression of two groups was detected by real time-PCR. Results The A4galT gene expression increased in the chitosan coated culture dish. Conclusion Chi.tosan has a good biocompatibility with DRG cell, and is a cheap biomaterial for cell culture dish in laboratory.

关 键 词：壳聚糖 背根神经节 

分 类 号：R741[医药卫生—神经病学与精神病学]