高效液相色谱测定肉及肉制品中的4种添加剂  

Simultaneous determination of four additives in meat and meat products by using high performance liquid chromatography

作　　者：米尔芳[1] 王彩云[1] 邓俊平[1] 

机构地区：[1]山西省疾病预防控制中心,太原030012

出　　处：《中国卫生检验杂志》2008年第7期1337-1338,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：采用水提取，甲醇低温沉淀蛋白，水定容离心过滤的前处理方法，使用高效液相色谱法同时测定乙酰磺胺酸钾、糖精钠、苯甲酸、山梨酸。结果：其精密度分别为2．9％、3．1％、2．6％、2．8％，最低检测浓度分别为0．005、0．005、0．005、0．0025g／kg，方法线性r〉0．9995，回收率均在90％～98％之间。结论：该方法简便快捷，准确度高，适用于大批量样品的快速分析。Objectives.To establish a method for determination of acesulfame potassium saccharin sodium benzoic acid, sorbic acid in meat and meat products by HPLC. The preparation included water extracting precipitatting protein by metyl alcohol at low temperature and centrifuging and tilting after fixed water volume. Result： Relative standard derivation of accesulfame potassium saccharin sodium benzoic acid,sorbic acid were respectively 2.9% ,3. 1% ,2.6% ,2.8% and the detection limit of them were respectively 0. 005,0. 005,0. 005,0. 0025 g/kg. A linear relationship holded over 0. 9995. Their recoveries were among 90% 98%. Conclusion：This method was simple,fast and accurate,and can be applied to the determination of a large of samples.

关 键 词：高效液相色谱 肉及肉制品 乙酰磺胺酸钾 糖精钠 苯甲酸 山梨酸 

分 类 号：O657.72[理学—分析化学]