石油降解细菌的筛选及培养条件的初步研究  

The Primary Study of Screening and The Culture Condition of The Degradation Bacteria by Petroleum

作　　者：慕庆峰[1] 洪音[1] 

机构地区：[1]黑龙江八一农垦大学植物科技学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2008年第3期19-22,共4页journal of heilongjiang bayi agricultural university

基　　金：校内科研启动资金资助。

摘　　要：从大庆油田分离纯化出7株能以石油作为唯一碳源的石油降解细菌,并对降解能力进行研究,发现各菌株均能产表面活性剂,其中油膜面积大于3cm的菌株有PW01、PS01、PS02、PS04、PS05 5株细菌,各菌株均能产酸。摇瓶培养菌株PW01 7d,发现其对石油降解率高达68.33%,最适氮源为NH4Cl、最适磷源为1∶1的KH2PO4/K2HPO4,在此基础上设计正交试验,极差R大小比较发现各因素对菌株PW01的石油降解率影响次序为:石油>KH2PO4/K2HPO4>NH4Cl。Seven predominant strains which use petroleum hydrocarbon as the sole carbon source for decomposing oil had been isolated from the oil-polluted soil of Daqing oil field. Then the competence of the degradation was studied. The results was as followed： every bacteria could produce biosurfactant and the oil velum bigger than 3 cm were five bacteria which were PW01,PS05,PS02,PS01 and PS04.All of them could produce acid. PW01 bacteria was shaking cultured for seven days, and its degradation rate arrived at 68.33 %,the proper nitrogen source was NH4Cl and phosphorus source was KH2PO4/K2HPO4=1：1, The orthogonal experiment was designed on the base, the effect of every factor on the oil degradation was petroleum〉KH2PO4/K2HPO4〉NH4Cl by the comparison of range.

关 键 词：菌株筛选 石油降解 正交设计 

分 类 号：X172[环境科学与工程—环境科学]