纤维素酶对黑大豆和葛根异黄酮糖苷不同组分的水解活性  

Specific hydrolysis of isoflavone glycosides of black soybean and kudzu using cellulase

作　　者：李丹[1,2] 李晓磊[1] 谭克[1] 

机构地区：[1]长春大学吉林省农产品加工生物技术重点实验室,长春130022 [2]国家大豆深加工技术研究推广中心,长春130022

出　　处：《食品科技》2008年第7期26-30,共5页Food Science and Technology

基　　金：国家科技成果重点推广计划项目(2003EC000127);吉林省科技发展计划项目(20050125)

摘　　要：异黄酮主要以糖苷的分子形式存在于黑大豆和葛根中,但是其具有生物活性的部分主要是苷元。为了建立一种体外生物转化异黄酮糖苷为异黄酮苷元的新方法,采用70%乙醇提取黑大豆和葛根异黄酮,用高效液相色谱分析了纤维素酶水解前后两粗提物的异黄酮组成。结果表明:纤维素酶对于不同种类的异黄酮糖苷具有不同的水解专一性、基团专一性和键专一性,能完全水解大豆苷和染料木苷为大豆素和染料木素,而不能水解丙二酰基染料木苷、丙二酰基大豆苷和葛根素。Isoflavones occurred in black soybean and kudzu are mainly as glycosides. However, their bioactivities lie in the aglycones. Cellulase was used to convert isoflavone glycosides extracted from black soybean and kudzu with 70% ethanol into the aglycones in this study. The compositions of isoflavone extract before and after cellulase treatment were analyzed by HPLC. The results showed that daidzin and genistin could be completely hydrolyzed into daidzein and genistein by cellulase. But the hydrolysis of malonyl daidzin, malonyl genistin and puerarin could not be found.

关 键 词：异黄酮 黑大豆 葛根 纤维素酶 

分 类 号：TS21[轻工技术与工程—粮食、油脂及植物蛋白工程]