茶树的RAPD标记向SCAR标记转化的研究  

Research on RAPD marker to SCAR marker in Camellia sinensis

作　　者：丁洲[1] 江昌俊[2] 叶爱华[1] 陈聪[1] 谭振[1] 张莉[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学茶与食品科技学院,合肥230036

出　　处：《安徽农业大学学报》2008年第3期315-318,共4页Journal of Anhui Agricultural University

基　　金：安徽省教育厅自然科学研究计划重点项目(2004kj137zd)资助

摘　　要：SCAR标记是在RAPD技术的基础上发展起来的一种新型分子标记技术,相对其他分子标记,它具有开发成本低,稳定性高,单位点多态等特点,将为茶树分子育种开辟一条新的有效途径。通过将随机引物在不同茶树品种基因组DNA中扩增得到的特异性片段,经回收、克隆、测序后重新设计1对特异性引物,将RAPD标记成功转化成SCAR标记,提高了分子鉴定的稳定性。SCAR marker is one of new molecular markers based on RAPD technology. It has such merit as low price, high stability, site-specific and so on compared with other markers, and itmay provide a new way. for molecular breeding of tea. In this experiment, the special segment with random primer in different tea cultivars genomic DNA was recovered, cloned and sequenced. According to its sequence, a pair of special primers was synthesized and the RAPD marker was successfully converted into SCAR marker. It improves stability of molecular identification and affirm applied prospect of SCAR technology in genetic resources of tea.

关 键 词：茶树 RAPD标记 SCAR标记 转化 

分 类 号：S571.1[农业科学—茶叶生产加工] Q78[农业科学—作物学]