采用基因芯片技术筛选黑斑息肉综合征相关基因  

Screening and annotation of genes associated with Peutz-Jeghers syndrome using gene chip technique

作　　者：戴益琛[1] 宋于刚[2] 谢军培[1] 曾伟[1] 

机构地区：[1]中国人民解放军174医院消化科,福建省厦门市361003 [2]南方医科大学南方医院消化科,广东省广州市510515

出　　处：《世界华人消化杂志》2008年第19期2191-2194,共4页World Chinese Journal of Digestology

摘　　要：目的:探索黑斑息肉综合征(Peutz-Jeghers syndrome,PJS)特异性相关基因.方法:用基因芯片技术研究PJS息肉和大肠腺瘤的基因表达谱,筛选出两者的差异表达基因,通过比较,建立和研究PJS特异的差异基因表达谱,并以RT-PCR对部分差异表达基因进行检测来验证芯片结果.结果:大肠PJS息肉组的270个特异性差异表达的基因,基因上调166个,下调104个.PJS息肉部分特异差异表达基因分别为:EPHB4、EPHB3、EPHB1、EFNB2、EFNA1、COL4A1、COL4A2、COL6A3和COL6A2.结论:Ephrin、COL4A1、COL4A2、COL6A2和COL6A3基因可能是PJS特异性相关基因.AIM： To explore the genes associated with Peutz-Jeghers syndrome （PJS） using gene chip technique. METHODS： Differentially expressed genes of PJS polyps and colorectal adenoma tissues were identificated using DNA microarray. Part of differentially expressed genes were identificated by semiquantitative reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS： In comparison with normal mucosa and adenoma, 270 genes were differentially expressed in PJS polyps, of which 166 were up- regulated and 104 were down-regulated. PJS- specific differently expressed genes included EPHB4, EPHB3, EPHB1, EFNB2, EFNA1, CO- L4A1, COL4A2, COL6A3 and COL6A2. CONCLUSION： Ephrin, COL4A1, COL4A2, COL6A2 and COL6A3 are the novel genes associated with PJS, and they may play important roles in the pathogenesis of PJS polyps.

关 键 词：黑斑息肉综合征 微阵列 基因 逆转录聚合酶链式反应 

分 类 号：R735.3[医药卫生—肿瘤]