藏药寨卡有效部位的分离鉴定及其抗肿瘤活性研究  

Preliminary Study on the Extraction of Active Fraction from the Seeds of Tibet Medicine Thlaspi arvense Linn.and its Antitumor Activities and its

作　　者：李艳[1] 江南[1] 罗霞[2] 清源[1] 许晓燕[2] 杨志荣[1] 

机构地区：[1]四川大学生命科学学院.四川大学生物资源与生态环境教育部重点实验室,四川成都610064 [2]四川省中医药研究院中药细胞与分子生物学实验室,四川成都610064

出　　处：《时珍国医国药》2008年第5期1118-1120,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的分离鉴定藏药寨卡的有效部位并对其抗肿瘤活性进行初步研究。方法采用甲醇萃取、酸性氧化铝层析柱层析和谱图分析对寨卡有效部位进行分离提取和鉴定,采用S180荷瘤小鼠模型和CT-26荷瘤小鼠模型,探讨寨卡有效部位提取物的抗肿瘤活性。结果提取分离得到了寨卡中有抗肿瘤活性的有效部位,并经谱图分析鉴定该部位为硫代葡萄糖苷类。经抑瘤实验表明,其能明显抑制S180荷瘤小鼠和CT-26荷瘤小鼠的肿瘤增殖。结论硫代葡萄糖苷是寨卡抑制肿瘤增殖的有效部位,具有一定的抗肿瘤活性。Objective To extract and identify the active fraction of the seeds of Tibet Medicine Thlaspi arvense Linn. and to study its antitumor activities. Methods The active fraction of Thlaspi arvense linn was extracted and identified by nethanol -extraction, tart - Al2O3 colum chromatography ,TLC and HPLC. The anti - tumor effect was observed on the mouse model of S180 AND CT - 26. Results The active fraction of Thcaspi aruense Linn. was glucosinolate, and it could evidently inhibit the tumor propagation of the two kinds of animal models. Conclusion Glucosinolate is the active fraction of Thlaspi arvense Linn and it has good antitumor effects.

关 键 词：寨卡 有效部位 硫代葡萄糖苷 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]