Intrahepatic CD8^+ lymphocyte trapping during tolerance induction using mushroom derived formulations:A possible role for liver in tolerance induction  

作　　者：Mony Shuvy Tiberiu Hershcovici Lidya Zolotarov Yaron Ilan Cristina LulI-Noguera Harry Wichers Ofer Danay Dan Levanon 

机构地区：[1]Liver Unit, Department of Medicine, Hadassah, HebrewUniversity Medical Center, Jerusalem IL-91120, Israel [2]WageningenUniversity and Research Center, Wageningen 6706 KN, TheNetherlands [3]Migal, Kiryat Shmone 11016,Israel

出　　处：《World Journal of Gastroenterology》2008年第24期3872-3878,共7页世界胃肠病学杂志（英文版）

基　　金：(in part) The Roman-Epstein Liver Research Foundation (to Y.I.)

摘　　要：AIM: To determine the immunomodulatory effect of Shiitake (a mushroom extract),we tested its effect on liver-mediated immune regulation in a model of immune-mediated colitis. METHODS: Four groups of mice were studied. Colitis was induced by intracolonic instillation of TNBS in groups A and B. Groups A and C were treated daily with Shiitake extract,while groups B and D received bovine serum albumin. Mice were evaluated for development of macroscopic and microscopic. The immune effects of Shiitake were determined by FACS analysis of intra-hepatic and intrasplenic lymphocytes and IFN-γ ELISPOT assay. RESULTS: Administration of Shiitake resulted in marked alleviation of colitis,manifested by signifi cant improvement in the macroscopic and microscopic scores,and by reduction in IFN-γ-producing colonies in group A,compared to group B mice (1.5 pfu/mL vs 3.7 pfu/mL,respectively). This beneficial effect was associated with a significant increase in the intra-hepatic CD8+ lymphocyte trapping,demonstratedby an increased intrasplenic/intrahepatic CD4/CD8 lymphocyte ratio. These effects were accompanied by a 17% increase in the number of intrahepatic natural killer T (NKT) cells. A similar effect was observed when Shiitake was administered to animals without disease induction. CONCLUSION: Shiitake extract affected liver-mediated immune regulation by altering the NKT lymphocyte distribution and increasing intrahepatic CD8+ T lymphocyte trapping,thereby leading to alleviation of immune-mediated colitis.AIM： To determine the immunomodulatory effect of Shiitake （a mushroom extract）, we tested its effect on liver-mediated immune regulation in a model of immune-mediated colitis. METHODS： Four groups of mice were studied. Colitis was induced by intracolonic instillation of TNBS in groups A and B. Groups A and C were treated daily with Shiitake extract, while groups B and D received bovine serum albumin. Mice were evaluated for development of macroscopic and microscopic. The immune effects of Shiitake were determined by FACS analysis of intra-hepatic and intrasplenic lymphocytes and IFN-γ ELISPOT assay. RESULTS： Administration of Shiitake resulted in marked alleviation of colitis, manifested by significant improvement in the macroscopic and microscopic scores, and by reduction in IFN-γ-producing colonies in group A, compared to group B mice （1.5 pfu/mL vs 3.7 pfu/mL, respectively）. This beneficial effect was associated with a significant increase in the intrahepatic CD8^＋ lymphocyte trapping, demonstratedby an increased intrasplenic/intrahepatic CD4/CD8 lymphocyte ratio. These effects were accompanied by a 17% increase in the number of intrahepatic natural killer T （NKT） cells. A similar effect was observed when Shiitake was administered to animals without disease induction. CONCLUSION： Shiitake extract affected livermediated immune regulation by altering the NKT lymphocyte distribution and increasing intrahepatic CD8^＋ T lymphocyte trapping, thereby leading to alleviation of immune-mediated colitis.

关 键 词：MUSHROOMS Colitis Immune modulation SHIITAKE Natural killer T cell 

分 类 号：R575[医药卫生—消化系统]