shRNA对人白血病K562饴LDM细胞多药耐药性的逆转观察  

The complete reversal of mulitidrug resistance in leukemia cells by shRNA

作　　者：张雪琴[1] 蔡卫东[1] 

机构地区：[1]深圳市宝安区松岗人民医院儿科,广东省深圳518105

出　　处：《中国基层医药》2008年第5期788-790,883,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨利用RNA干扰（RNAi）技术来逆转人白血病K562／ADM细胞多药耐药能力的可行性。方法设计两条针对MDR1基因的发夹状RNA（shRNA），并将其分别构建在pGenSil-1质粒中；然后将这两条shRNA分别转染K562／ADM。利用罗丹明外排法、荧光定量PCR来检测这两条shRNA对K562／ADM的多药耐药性的逆转作用。结果在稳定转染的细胞中可以观察到不同程度的多药耐药（MDR）逆转现象。在两个稳定转染的克隆中，MDR现象被完全逆转。结论本研究显示可以利用shRNA逆转K562／ADM细胞的多药耐药问题。Objective To study the efficiency of reversing MDR by suppressing MDR1 gene with RNAi in K562/ADM cells. Methods For reversing MDR by RNAi technology,two different short hairpin RNAs（shRNAs） were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into the highly adri- amycin-resistant K562/ADM cell line. The RNAi effect on MDR was evaluated by real-time PCR, and Rho- damine123（RM23） efflux assy. Results The stable transfected clones showed varied degrees of reversal of MDR phenotype. Surprisingly,the MDR phenotype was completely reversed in two transfected clones. Conclusion This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells,which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs.

关 键 词：多药耐药相关蛋白类 基因 MDR RNA干扰 白血病 

分 类 号：R733[医药卫生—肿瘤]