N^+注入诱变筛选阿拉伯糖利用缺陷型肌苷高产菌株  

Screening of a High Inosine-producing Mutant with Arabinose Utilization Defi ciency by N^+ Ion Implantation

作　　者：刘国生[1] 赵婷[1] 王秀强 李宗义[1] 秦广雍[3] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡拓新生化科技有限公司,河南新乡453000 [3]河南省离子束生物工程重点实验室,河南郑州450052

出　　处：《中国医药工业杂志》2008年第7期504-506,共3页Chinese Journal of Pharmaceuticals

基　　金：河南省教育厅科技攻关项目(200761004);河南省高校青年骨干教师资助项目(2005046)

摘　　要：以枯草芽孢杆菌(Bacillus subtilis)HSD06为出发菌株发酵制备肌苷,注入不同剂量的N+离子束选育不能利用阿拉伯糖的突变株。结果表明低剂量和较低的致死率利于正突变株的筛选。当照射剂量为2×1014ions/cm2时,获得肌苷高产菌株B67,肌苷的摇瓶发酵产量为15.12g/L,比出发菌株提高了18.6%。Different N^＋ ion doses were implanted in an inosine-producing Bacillus subtilis HSD06 and the arabinose utilization deficiency mutants were screened. The result showed that lower dose and lethality rate were effective for screening of positive mutations. When the implantation dose was at 2×10^14 ions/cm^2, a high inosine-producing strain B67 was obtained. The yield of inosine by strain B67 in the shake flask reached to 15.12g/L, 18.6 % more than that of the initial strain.

关 键 词：肌苷 离子注入 枯草芽孢杆菌 阿拉伯糖利用缺陷型突变株 筛选 

分 类 号：TQ920[轻工技术与工程—发酵工程]