溴甲酚紫光度法测定水果的抗氧化活性  

SPECTROPHOTOMETRIC DETERMINATION OF ANTIOXIDATIVE ACTIVITY OF FRUIT BY BROMOCRESOL PURPLE DECOLOURATION

作　　者：丁宗庆[1] 李小玲[2] 

机构地区：[1]郧阳师范高等专科学校化学系,湖北丹江口442700 [2]湖北中医学院医学检验与技术系,湖北武汉430064

出　　处：《食品研究与开发》2008年第3期54-57,共4页Food Research and Development

摘　　要：研究了用溴甲酚紫做显色剂分光光度法测定Fenton(芬顿)反应产生羟自由基的方法。产生的羟自由基与溴甲酚紫作用使溶液吸光度降低,利用吸光度的变化间接测定所产生的羟自由基,测定了10种水果的抗氧化活性,其中猕猴桃的活性最强,羟自由基清除率达76.3%。A new method was developed for determination of the hydroxyl radical in Fenton reaction by spectrophotometry. Bromocresol purple react with hydroxyl radical, then the absorbency of the solution was declined. It can be used to determine the hydroxyl radical indirectly due to the change of absorbency. The antioxidant activities of ten kinds of fruits was thus determined. The results showed that the antioxidant activity of Chinese goosebeery was the strongest. It eradication rate to hydroxyl radical was 76.3 %.

关 键 词：溴甲酚紫 羟自由基 抗氧化活性 水果 

分 类 号：TS255.7[轻工技术与工程—农产品加工及贮藏工程]