小鼠肝癌细胞H_(22)培养上清液对鼠细胞L_(929) MTP53、EGFR蛋白表达的影响  

The Effects of Supernatant of Mice Hepatocellular Carcinoma Cells H_(22) on the Expressions of MTP53 and EGFR of L_(929) Cells

作　　者：张晓艳[1] 田芳[1] 汤为学[2] 

机构地区：[1]郑州大学基础医学院病理生理学教研室,河南郑州450052 [2]重庆医科大学病理生理学教研室,重庆400016

出　　处：《肿瘤基础与临床》2008年第4期296-297,共2页journal of basic and clinical oncology

摘　　要：目的探讨小鼠肝癌细胞H22培养上清液对鼠L929细胞MTP53、EGFR蛋白表达的影响。方法采用肝癌细胞H22的培养上清液诱导正常成纤维细胞L929,诱导细胞为L929-H22。采用免疫细胞化学方法检测MTP53、EGFR蛋白的表达。结果MTP53、EGFR蛋白的表达L929-H22细胞较L929细胞显著增强(Ρ<0.001)。结论小鼠肝癌细胞培养上清液诱导细胞MTP53、EGFR的表达与正常细胞不同,MTP53、EGFR的表达增强可能在细胞恶性转化过程中发挥作用。Objective To investigate the expressions of MTP53 and EGFR of normal cells and cells induced with the supernatant of mice hepatocellular carcinoma cells and the probable mechanism. Methods The normal cells L929 was induced with the supernatant of mice hepatocellular carcinoma cells H22 and named the induced cells as L929-H22 cells. The immunocytochemistry assay was used to detect the expressions of MTP53 and EGFR in L929 and L929 -H22 cells. Results Compared With L929 cells, L929 -H22 cells showed significantly higher expression level of MTP53 and EGFR （P 〈 0. 001 ）. Conclusion The expressions of MTP53 and EGFR in cells induced with supernatant of mice hepatocellular carcinoma cells were different from those in normal cells. The higher expressions of MTP53 and EGFR may play an important role in the carcinogenesis.

关 键 词：肝癌细胞H22 培养上清液 诱导 MTP53 EGFR 成纤维细胞L929 

分 类 号：R73-37[医药卫生—肿瘤] R735.7[医药卫生—临床医学]