水稻细胞质雄性不育育性回复株的基因型鉴定  

Identification of Genotype of Fertility Reversion Plants Occurring in Rice CMS Line

作　　者：文建成[1] 黄大军[1] 谭学林[1] 王石华[1] 谭亚玲[1] 顾晓明[1] 李伟华[1] 房毅[1] 

机构地区：[1]云南农业大学稻作研究所,昆明650201

出　　处：《分子植物育种》2008年第4期650-654,共5页Molecular Plant Breeding

基　　金：教育部博士点基金项目(20070676001);国家科技支撑计划(2006BAD01A01-7);云南省十一五科技攻关项目(2006NG05)资助

摘　　要：通过对在套袋繁殖产生的滇Ⅰ型不育系合系42A群体中发现的可育株、及其与不育系杂交产生的F1和可育株自交S1代的育性和核恢复基因位点分析,发现不育系合系42A中出现的大约0.11%可育株包括两种类型。一类细胞质正常可育,核无恢复基因,基因型为N(rf/rf),类似保持系,认为是保持系混杂所致。另一类可育株的细胞质雄性不育,核有恢复基因,其恢复基因位于水稻Rf-1基因区域,基因型为S(Rf/rf)。这类可育株不可能来自异品种或保持系串粉,可能是细胞核携带的育性相关基因发生育性自然回复突变导致。本研究将这类可育株简称为"育性回复株"。About 0.11% fertile plants discovered in a CMS-D1 line Hexi42A population that created by covering with bagged paper were grouped into two types through analyzing fertility and nuclear Rf locus of those plants as well as individuals in their S1 progenies of self-pollinated and F1 populations of cross with Hexi42A as the female parent. One type is genotyping N （rf/rf） which same as that of maintainer line. It is considering derived from contamination of maintainer line seeds in the process of CMS lines multiplication or management. Another one is genotyping S （Rf/rf） which contains nuclear Rf gene. The gene that located at Rf-1 locus of rice could not be pollinated by containing Rf gene pollens of off-type varieties or isocytoplasmic maintainer line. It assumed that the nuclear Rf allele of these fertile plants could be occurred naturally. This type was named fertility reversion plants of male sterility in this paper.

关 键 词：滇Ⅰ型细胞质雄性不育系 可育株 恢复基因 育性 

分 类 号：S511[农业科学—作物学]