SMMC-7721细胞线粒体DNA D-loop区点突变的研究  

Point mutation within D-loop region of mitochondrial DNA in human liver cancer cell line SMMC-7721

作　　者：王洁[1] 王学敏[2] 缪明永[2] 

机构地区：[1]南京军区南京总医院检验研究所临床中心实验科,南京210002 [2]上海第二军医大学生物化学与分子生物学教研室,200433

出　　处：《临床肿瘤学杂志》2008年第7期577-579,共3页Chinese Clinical Oncology

基　　金：国家自然科学基金项目(C30171030,C30472175)

摘　　要：目的:分析人肝癌细胞株SMMC-7721细胞线粒体DNA(mitochondrial DNA,mtDNA)D-loop区点突变的情况。方法:应用改良一步法提取mtDNA,PCR扩增D-loop区,产物经筛选后测序。结果:在SMMC-7721细胞mtDNA D-loop区发现三处新的点突变位点,分别是16166nt(A→G)、16278nt(C→T)和16362nt(T→C)。结论:SMMC-7721细胞mtDNA D-loop区新发现的点突变可能与肿瘤的发生发展有关。Objective：To detect the point mutation of D-loop region of mitochondrial DNA （mtDNA） in human liver cancer cell line SMMC-7721. Methods：mtDNA was extracted by improved one-step method, D-loop fragments were amplified by PCR, the PCR products were determined by DNA sequencing after blue-white selection. Results：Three new sites of point mutation within the D- loop region of mtDNA, 16 166nt（A→G）, 16 278nt（C→T） and 16 362nt（T→C）, were found in SMMC-7721. Conclusion：These point mutations might play an important role in the occurrence and development of carcinomas.

关 键 词：SMMC-7721细胞 MTDNA D—loop区 点突变 

分 类 号：R73-35[医药卫生—肿瘤]