Natural jasmonates of different structures suppress the growth of human neuroblastoma cell line SH-SY5Y and its mechanisms  

作　　者：Qiang-song TONG Guo-song JIANG Ll-duan ZHENG Shao-tao TANG Jia-bin CAI Yuan LIU Fu-qing ZENG Ji-hua DONG 

机构地区：[1]Departments of Surgery [2]pathology,Huazhong University of Science and Technology, Wuhan 430022, China [3]Central Laboratory, Huazhong University of Science and Technology, Wuhan 430022, China

出　　处：《Acta Pharmacologica Sinica》2008年第7期861-869,共9页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China （No 30200284, 30600278, and 30772359） and the Program for New Century Excellent Talents in University of China （NCET-06-0641 ）.

摘　　要：Aim： Recent evidence has indicated that members of natural jasmonates, a family of plant stress hormones, exhibit anticancer activity. The current study was undertaken to investigate the effects of jasmonates on the in vitro growth of human neuroblastomas, one of the most common solid tumors in children. Methods： Cellular proliferation was measured by 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide colorimetry and colony formation assay. Apoptosis was detected by Hoechst 33258 staining and flow cytometry. Western blotting was applied to assay gene expression. Results： The administration of natural jasmonates, methyl jasmonate, cis-jasmone, and jasmonic acid to cultured neuro- blastoma cell line SH-SY5Y, resulted in a decrease of cell proliferation in a doseand time-dependent manner. However, the in vitro growth of cultured human embryonic kidney （HEK） cell line HEK 293 was not affected by jasmonates. The cell cycles of jasmonate-treated SH-SY5Y cells were arrested at the G2/M phase. The incubation of SH-SY5Y cells with jasmonates resulted in characteristic changes of apoptosis. The anticancer activities of natural jasmonates on SH-SY5Y cells are as follows： methyl jasmonate〉cis-jasmone〉jasmonic acid. In addition, the expressions of proliferating cell nuclear antigen and N-myc were downregulated by methyl jasmonate. Moreover, methyl jasmonate decreased the expression of the X- linked inhibitor of apoptosis protein and survivin, critical members of inhibitors of the apoptosis protein family, in SH-SY5Y cells. Conclusion： Jasmonates suppress the growth of human neuroblastoma cell line SH-SY5Y via inhibiting cell proliferation and inducing apoptosis, which lays the groundwork for further investigation into the anticancer activities and its mechanisms of natural jasmonates on human neuroblastomas.Aim： Recent evidence has indicated that members of natural jasmonates, a family of plant stress hormones, exhibit anticancer activity. The current study was undertaken to investigate the effects of jasmonates on the in vitro growth of human neuroblastomas, one of the most common solid tumors in children. Methods： Cellular proliferation was measured by 3-（4,5-dimethylthiazol-2-yl）-2,5- diphenyltetrazolium bromide colorimetry and colony formation assay. Apoptosis was detected by Hoechst 33258 staining and flow cytometry. Western blotting was applied to assay gene expression. Results： The administration of natural jasmonates, methyl jasmonate, cis-jasmone, and jasmonic acid to cultured neuro- blastoma cell line SH-SY5Y, resulted in a decrease of cell proliferation in a doseand time-dependent manner. However, the in vitro growth of cultured human embryonic kidney （HEK） cell line HEK 293 was not affected by jasmonates. The cell cycles of jasmonate-treated SH-SY5Y cells were arrested at the G2/M phase. The incubation of SH-SY5Y cells with jasmonates resulted in characteristic changes of apoptosis. The anticancer activities of natural jasmonates on SH-SY5Y cells are as follows： methyl jasmonate〉cis-jasmone〉jasmonic acid. In addition, the expressions of proliferating cell nuclear antigen and N-myc were downregulated by methyl jasmonate. Moreover, methyl jasmonate decreased the expression of the X- linked inhibitor of apoptosis protein and survivin, critical members of inhibitors of the apoptosis protein family, in SH-SY5Y cells. Conclusion： Jasmonates suppress the growth of human neuroblastoma cell line SH-SY5Y via inhibiting cell proliferation and inducing apoptosis, which lays the groundwork for further investigation into the anticancer activities and its mechanisms of natural jasmonates on human neuroblastomas.

关 键 词：JASMONATE NEUROBLASTOMA proliferating cell nuclear antigen N-MYC X-linked inhibitor of apoptosis protein： survivin 

分 类 号：R36[医药卫生—病理学]