Microinjection of resveratrol into rostral ventrolateral medulla decreases sympathetic vasomotor tone through nitric oxide and intracellular Ca^2＋ in anesthetized male rats^1  

作　　者：Hui-juan MA Ya-kun CAO Yi-xian LIU Ru WANG Yu-ming WU 

机构地区：[1]Department of Physiology, Hebei Medical University, Shijiazhuang 050017, China

出　　处：《Acta Pharmacologica Sinica》2008年第8期906-912,共7页中国药理学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China （No 304005）; New Century Excellent Talents Foundation of Ministry Enducation of China （No NCET-07-0252）; the Natural Science Foundation of Hebei Province （No 303467）, the Science and Technology Department of Hebei Province （No 062761383）, and The Administration of Traditional Chinese Medicine of Hebei Province （No 2006197）.

摘　　要：Aim： To define the effect of resveratrol （RES） on the central regulation of blood pressure （BP）, heart rate （HR）, and renal sympathetic nerve activity （RSNA）. Methods： RES was microinjected into the rostral ventrolateral medulla （RVLM）, and BP, HR, and RSNA were recorded simultaneously in anesthetized rats. Results： A rnicroinjection of RES （20, 40, and 80 μmol/L, 0.2μL） into the RVLM dose dependently decreased BP, HR, and RSNA. Pretreatment with an anti-estrogen tamoxifen （100 μmol/L, 0.2 μL） did not affect the effects of RES. Pretreatment with N^G-nitro-L-arginine methyl ester （100 μmol/L, 0.2 μL）, an inhibitor of nitric oxide （NO） synthase, could completely abolish the effect of RES. A prior microinjection of Bay K8644 （500 nmol/L, 0.2 μL）, an agonist of calcium channels, could also abrogate the effect of RES. Prior administration of a potent inhibitor of tyrosine phosphatase, sodium orthovanadate （1 mmol/L, 0.2 μL）, could partially attenuate the inhibitory effect of RES. Conclusion： The results suggest that a microinjection of RES into the RVLM inhibits BP, HR, and RSNA. The effects may be mediated by NO synthesis and a decrease in Ca^2＋ influx, in which protein tyrosine kinase is involved.Aim： To define the effect of resveratrol （RES） on the central regulation of blood pressure （BP）, heart rate （HR）, and renal sympathetic nerve activity （RSNA）. Methods： RES was microinjected into the rostral ventrolateral medulla （RVLM）, and BP, HR, and RSNA were recorded simultaneously in anesthetized rats. Results： A rnicroinjection of RES （20, 40, and 80 μmol/L, 0.2μL） into the RVLM dose dependently decreased BP, HR, and RSNA. Pretreatment with an anti-estrogen tamoxifen （100 μmol/L, 0.2 μL） did not affect the effects of RES. Pretreatment with N^G-nitro-L-arginine methyl ester （100 μmol/L, 0.2 μL）, an inhibitor of nitric oxide （NO） synthase, could completely abolish the effect of RES. A prior microinjection of Bay K8644 （500 nmol/L, 0.2 μL）, an agonist of calcium channels, could also abrogate the effect of RES. Prior administration of a potent inhibitor of tyrosine phosphatase, sodium orthovanadate （1 mmol/L, 0.2 μL）, could partially attenuate the inhibitory effect of RES. Conclusion： The results suggest that a microinjection of RES into the RVLM inhibits BP, HR, and RSNA. The effects may be mediated by NO synthesis and a decrease in Ca^2＋ influx, in which protein tyrosine kinase is involved.

关 键 词：RESVERATROL renal sympathetic nerveactivity estrogen receptor nitric oxide Ca^2＋ protein tyrosine kinase 

分 类 号：R36[医药卫生—病理学]