Expression of the retinoic acid-metabolizing enzymes RALDH2 and CYP26b1 during mouse postnatal testis development  

作　　者：Jing-Wen Wu Ru-Yao Wang Qiang-Su Guo Chen Xu 

机构地区：[1]Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China [2]Shanghai Key Laboratory for Reproductive Medicine, Shanghai 200025, China

出　　处：《Asian Journal of Andrology》2008年第4期569-576,共8页亚洲男性学杂志（英文版）

基　　金：We thank Dr Bing-Shi Guo for the linguistic revision of the manuscript, and Jin-Mei Wang for her excellent technical assistance. This work was supported by the National Natural Science Foundation of China （No. 30070391） and the Fourth Shanghai Municipal Education Commission Key Academic Discipline Foundation, China （No. ZDXK 2001）.

摘　　要：Aim： To study the expression pattern of the retinoic acid metabolizing enzymes RALDH2 and CYP26bl during mouse postnatal testis development at both mRNA and protein levels. Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood.Aim： To study the expression pattern of the retinoic acid metabolizing enzymes RALDH2 and CYP26bl during mouse postnatal testis development at both mRNA and protein levels. Methods： Real-time polymerase chain reaction and Western blot analysis were performed to determine the relative quantity of RALDH2 and CYP26bl at both mRNA and protein levels at postnatal day 1, 5, 10, 20, and in adult mice （70 days testes）. Testicular localization of RALDH2 and CYP26b 1 during mouse postnatal development was examined using immunohistochemistry assay. Results： Aldhla2 transcripts and its protein RALDH2 began to increase at postnatal day 10, and remained at a high level through postnatal day 20 to adulthood. Cyp2661 transcripts and CYP26bl protein did not change significantly during mouse postnatal testis development. RALDH2 was undetectable in the postnatal 1, 5 and 10 day testes using immunohis- tochemistry assay. At postnatal day 20 it was detected in pachytene spermatocytes. Robust expression of RALDH2 was restricted in round spermatids in the adult mouse testis. In the developing and adult testis, CYP26bl protein was confined to the peritubular myoepithelial cells. Conclusion： Our results indicate that following birth, the level of retinoic acid in the seminiferous tubules might begin to increase at postnatal day 10, and maintain a high level through postnatal day 20 to adulthood.

关 键 词：RALDH2 CYP26b1 retinoic acid SPERMATOGENESIS TESTIS 

分 类 号：R339.21[医药卫生—人体生理学]