香菇漆酶的纯化及部分性质研究  

Purification and Partial Characterzation of Laccase from Lentinus edodes

作　　者：王方忠[1] 朱启忠[1] 董学卫[1] 林金飞[1] 曲悦文[1] 徐国英[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209

出　　处：《北方园艺》2008年第6期36-38,共3页Northern Horticulture

摘　　要：对香菇漆酶进行了丙酮分级沉降、半透膜透析、DEAE-纤维素及Sephadex G100柱层析纯化，并研究了漆酶的产酶曲线及酶作用最适条件。结果表明：在该培养条件下，香菇第10天达产酶高峰，峰值酶活为216U/mL，酶作用的最适pH值为3.6，在pH值3.6-4.6有较强的稳定性。最适酶解温度为30℃，Ba2＋、Cu2＋对香菇漆酶有激活作用，而Ag＋、Fe3＋则能明显抑制漆酶活性，其它离子影响相对较小。以邻联甲苯胺为底物的表观Km值为1.68mmol/L。The laccase of Lentinus edodes was purified by classification deposition of acetone, dialyzed by semipermeable membrane, DEAE-- Cellulose ion exchange and Sephadex G100 column chromatography. The curve of laccase production and optimal condation of laccase reaction were also studied. The results showed that Lentinus edodes came the laccase production peak which was 216 U/mL at the 10 day in this culture condition. The optimal temperature and optimal pH of laccase reaction were at 30℃ and pH 3. 6. The laccase was kept strongly active when pH ranged from 3.6 to 4. 6. Ba^2＋ ,Cu^2＋ increased laccase activity whereas Ag^＋ ,Fe^3＋ obviously inhibited it. The form view Km of laccase was 1.68 mmol/L with o--Tolidine as substrate.

关 键 词：香菇 漆酶 纯化 酶学性质 

分 类 号：S646.12[农业科学—蔬菜学] Q946.5[农业科学—园艺学]