广西黄曲霉毒素B1高污染区210人CYP3A7基因多态性检测  

Polymorphisms of CYP3A7 Gene of 210 Persons in Guangxi with Heavy Aflatoxin B1 Contamination

作　　者：陆海霞[1] 冯震博[1] 冯小兰[1] 

机构地区：[1]广西医科大学病理学教研室,南宁530021

出　　处：《癌变．畸变．突变》2008年第4期292-294,共3页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:了解广西黄曲霉毒素B1(AFB1)高污染区人群细胞色素P450 3A7(CYP3A7)基因的多态性,及其基因型与肝细胞癌易感性之间的关系。材料与方法:选取黄曲霉毒素B1高污染区人群210例,其中肝细胞性肝癌患者97例,设为肝细胞性肝癌组(HCC组);HBV阳性而无其它疾病者38例,设为HBV阳性组;HBV阴性且无疾病者75例,设为HBV阴性组。取各组对象抗凝全血标本用酚-氯仿法提取DNA进行PCR扩增,并用限制性片段长度多态性检测CYP3A7*1A和CYP3A7*1C等位基因。结果:所检测的210份样本CYP3A7均为*1A/*1A型,未检测到*1C等位基因。结论:未检测到CYP3A7高表达*1C等位基因,推测CYP3A7在AFB1诱发的HCC发生中所起的作用小。BACKGROUND AND AIM： To investigate polymorphisms of CYP3A7 gene of population in Guangxi with heavy aflatoxin B1 （AFB1） contamination, and the relationship between CYP3A7 genotypes and susceptibility of hepatocellular carcinoma （HCC） . MATERIALS AND METHODS： Single nucleotide polymorphisms of CYP3A7＊IA and ＊ IC were analyzed in 210 cases and control samples （97 HCC, 38 HBV-positive but with no other disease and 75 HBV- negative no disease） using the polymerase chain reaction-restriction fragment length polymorphism assays. RESULTS： All of the samples were CYP3A7＊ IA/ IA genotype , CYP3A7＊ IC allele was not detected in this cohort of Guangxi population. CONCLUSION： CYP3AT＊IC allele was not detected in this cohort,suggesting that CYP3A7 might have a small influence on the development of AFBl-related HCC.

关 键 词：细胞色素P450 3A7遗传多态性 黄曲霉毒素B1 肝细胞性肝癌 

分 类 号：R730.45[医药卫生—肿瘤]