肿瘤相关基因fgfr3 mRNA在白血病细胞中的表达及其临床意义  

Expression of A Tumor Related Gene fgfr3 mRNA in Leukemic Cells and its Clinical Significance

作　　者：吴静怡[1] 黄亮[1] 熊婕[1] 曹阳[1] 孙立石[1] 刘文励[1] 周剑峰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《中国实验血液学杂志》2008年第4期738-741,共4页Journal of Experimental Hematology

摘　　要：本研究旨在了解白血病细胞中fgfr3基因的表达水平及其和临床的关系。应用RT-PCR法检测4个白血病细胞系及96例白血病患者和14例对照者骨髓样本中fgfr3mRNA的表达,并分析了其与临床指标及染色体异常的相关性。96例白血病患者包括36例AML,29例ALL和31例CML。结果表明:fgfr3基因在K562和U937细胞中有表达,而在HL-60和SHI-1细胞中无表达。AL组和CML组fgfr3基因的阳性率(分别为46.15%和51.61%)与对照组相比,差异有统计学意义(p<0.05)。AML组和ALL组fgfr3基因的阳性率(分别为44.44%和48.28%)均高于对照水平,差异有统计学意义(p<0.05)。fgfr3基因的阳性表达与外周血高白细胞计数(≥20×109/L)呈显著性正相关(p<0.05)。ALL患者中fgfr3基因的表达与bcr/abl融合基因的异常呈显著正相关(r=0.151,p<0.05)。而AL患者fgfr3基因的阳性表达与染色体预后分组无显著相关性。结论:AL和CML患者均存在fgfr3基因的过表达,提示fgfr3基因可能参与了AL和CML的发病。This study was aimed to explore fgfr3 gene expression in leukemic cells and its clinical significance. The expression levels of fgfr3 mRNA in 4 leukemic cell lines and bone marrow samples from 96 patients with leukemia and 14 controls were assayed by RT-PCR. 96 patients with leukemia included 36 cases of acute myeloid leukemia（ AML）, 29 cases of acute lymphoblastic leukemia（ALL）, 31 cases of chronic myelogenous leukemia （CML）. The results indicated that fgfr3 gene could be detected in both K562 and U937 cell lines, but not in HL-60 and SHI-1 cell lines. The positive expression rates of fgfr3 mRNA in AL and CML were 46.15% and 51.61% respectively, and were higher than that in control （ 14.29%, p 〈0.05 ）. The positive expression rates of fgfr3 mRNA in AML and ALL were 44.44% and 48.28% respectively, and were higher than that in control （p 〈 0.05 ）. The increased level of fgfr3 mRNA had a positive correlation with high white blood cell count of ≥20 × 10^9/L （p 〈 0.05 ）. Higher fgfr3 mRNA expression positively correlated with fuse genes of bcr/abl in ALL（ r =0. 151 ,p 〈0.05 ）. The expression of fgfr3 gene in AL was not related with chromosome abnormality. In conclusion, the over expression of fgfr3 gene exists in AL and CML patients, it suggests that fgfr3 gene may be partially involved in leukemogenesis.

关 键 词：白血病 肿瘤相关基因 FGFR3 MRNA K562 HL-60 U937 SHI-1 

分 类 号：R733.7[医药卫生—肿瘤]