二磷酸氯喹对K562细胞增殖与凋亡的影响  

Effects of Chloroquine Diphosphate on Proliferation and Apoptosis of Human Leukemic K562 Cells

作　　者：蒋培都[1] 赵瀛兰[1] 杨胜勇[1] 毛咏秋[1] 郑于珠[1] 李争光[1] 魏于全[1] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室

出　　处：《中国实验血液学杂志》2008年第4期768-771,共4页Journal of Experimental Hematology

摘　　要：本研究探讨二磷酸氯喹对K562细胞增殖与凋亡的影响及其可能的作用机制。用细胞增殖试验(MTT法)检测不同浓度二磷酸氯喹对K562细胞的增殖抑制作用;应用细胞形态学检查、流式细胞术、DNA琼脂糖凝胶电泳观察和检测细胞凋亡;以罗丹明123(Rhodam ine 123)为细胞染色剂,采用流式细胞术检测不同浓度二磷酸氯喹处理后K562细胞线粒体膜电位(ΔΨm)的变化。结果表明:用不同浓度二磷酸氯喹(1.5625、3.125、6.25、12.5、25、50、100μmol/L)分别作用于K562细胞24、48和72小时后,细胞生长活力明显降低,具有剂量依赖性;典型的细胞形态学改变、亚G1峰的测出、梯形条带的显现共同证实了二磷酸氯喹能诱导K562白血病细胞凋亡;Rhodam ine 123染色显示二磷酸氯喹处理的K562细胞线粒体膜电位强度下降。结论:二磷酸氯喹对K562细胞有生长抑制、诱导凋亡作用,其作用可能与细胞线粒体膜电位(ΔΨm)下降有关。The purpose of this study was to investigate the effects of chloroquine diphosphate on the proliferation and apoptosis of human leukemic K562 cells, and to elucidate its possible mechanism of activity. The inhibitory effect of chloroquine diphosphate with different concentrations on K562 cell proliferation was detected by MTT method. Apoptosis was measured by flow cytometry （FCM） ; morphological analysis of apoptosis was performed after staining with propidium iodide（PI） under fluorescence microscope; cell apoptosis was assessed by the DNA ladder shown agarose gel electrophoresis. After treatment with chloroquine diphosphate, K562 cells were stained by Rhodamine 123 to detect changes in mitoehondrial transmembrane potential（ΔΨm） by FCM. The results showed that the cell viability decreased in dose- dependent manner, following chloroquine diphosphate treatment at different concentrations （ 1. 5625, 3. 125, 6. 25, 12. 5, 25, 50 and 100 μmol/L） for 24, 48 and 72 hours. By FCM analysis, the significant increases of sub-G1 were observed. DNA ladder was detected and apoptotic nuclei were observed. ΔΨm decreased in K562 cells after chloroquine diphosphate treatment. It is concluded that the chloroquine diphosphate can inhibit the proliferation of K562 cells and induce cell apoptosis, which may relate to down-regulation of mitochonddal transmembrane potential （ΔΨm）.

关 键 词：二磷酸氯喹 K562细胞 细胞增殖 细胞凋亡 线粒体跨膜电位 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]