猪静脉桥血管重建中平滑肌细胞和成纤维细胞表型转化  

Phenotypic Modulation of Vascular Smooth Muscle Cells and Fibroblasts in Vascular Remodeling of Pig Vein Grafts

作　　者：邱志兵[1] 陈鑫[1] 万松[2] 

机构地区：[1]南京医科大学附属南京第一医院心胸外科,江苏省南京市210006 [2]香港中文大学威尔斯亲王医院心胸外科

出　　处：《中国动脉硬化杂志》2008年第7期532-536,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的动态观察静脉桥再狭窄动物模型中血管平滑肌细胞和外膜成纤维细胞表型转化和增殖活性的变化,评价血管平滑肌细胞和外膜成纤维细胞表型转化及增殖迁移对血管重塑的影响。方法建立猪静脉桥再狭窄模型,采用血管病理形态学和免疫组织化学方法,观察术后7、14和30d血管重塑及血管壁中增殖细胞核抗原、平滑肌α肌动蛋白和骨桥蛋白表达变化。结果①术后7d新生内膜形成并逐渐增厚,于术后30d达最大;内弹力板围绕面积于术后7d逐渐减小,术后30d管腔面积最小(P<0.05);重塑指数和外弹力板围绕面积术后7d稍有增大,其后不断减小,术后14～30d明显减小(P<0.05)。②增殖细胞核抗原染色发现,血管外膜成纤维细胞和血管平滑肌细胞增殖指数术后7d显著增加,术后14d增殖细胞核抗原阳性表达达高峰,术后30d回到基线水准。③平滑肌α肌动蛋白染色发现,术后7d中膜阳性面积减少,外膜和内膜中出现阳性表达;术后14d中膜血管平滑肌细胞阳性表达显著减少,外膜成纤维细胞和内膜中阳性表达显著增加;术后30d中膜血管平滑肌细胞阳性表达恢复,外膜阳性表达较术后14d和7d明显减少,内膜阳性表达较术后14d变化不明显。④骨桥蛋白染色发现,术后7d中膜血管平滑肌细胞阳性表达明显;术后14d中膜血管平滑肌细胞阳性表达较术后7d减少,而内膜中血管平滑肌细胞阳性表达显著增加且达高峰;术后30d中膜血管平滑肌细胞有少部分阳性表达,内膜中血管平滑肌细胞阳性表达较术后14d减少;外膜在术后各时间点均呈阴性表达。结论血管平滑肌细胞和成纤维细胞的表型转化和增殖活性改变对血管重塑起重要作用,参与并促进了血管桥再狭窄的发生过程。Aim To investigate the phenotypie modulation and proliferation changes of vascular smooth muscle cell （VSMC） and vascular adventitia fibroblasts （VAF） in the vein graft restenosis model. To evaluate the effects of the phenotypie modulation and proliferation changes of VSMC and VAF in vascular remodeling. Methods To create the pig autogenous vein grafts reatenosismodel, specimens were harvested at three fime points that was 7th, 14th and 30th day. Histomorphometrical and immunohemical approach were used to detect the changes of vascular remodeling and the expression of proliferation cell nuclear （PCNA）, smooth muscle α-actin （SM-α-actin） and osteopontin （OPN）. Results （1）At 7th day after operation, the neointima formed and continuously thickened, whose thickness reached maximum at 30th day postoperation. Internal elastic lamina （IELA） gradually decreased after operation, and at 30th day group luminal area reduced minimally （ P 〈 0.05）. Remodeling index and external elastic lamina （EEIA） slightly increased at 7th day group, but reduced gradually after that, which at 14th day and 30th day group decreased distinctly （ P 〈 0.05 ）. （2） According to PCNA stain, the proliferation index of VAF and VSMC increased significantly at 7th day after operation, which reached peak at 14th day, whereas the expression of PCNA reverted toward the baseline at 30th day after operation. （3）According to SM-α-actin stain, at 7th day after operation, the positive cell area decreased in the medium, but adventitia and neointimal were positive; At 14th day, the positive cell area decreased significantly in the medium, however, the positive area of adventitia and neointimal increased signiticantly; At 30th day positive expression of the medium recovered gradually, and the positive area of adventitia was less than that at 14th day, but the positive expression in the neointimal has no significant changes compared with 14th day. （4）OPN stain showed that the positive expression was signiti

关 键 词：病理学与病理生理学 细胞增殖 血管平滑肌细胞 成纤维细胞 表型 血管重塑 再狭窄 

分 类 号：R363[医药卫生—病理学]